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The potential role of HIV-specific CD38-/HLA-DR+ CD8+ T cells in viral suppressive activity and cytotoxicity in HIV controllers

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HAL Id: inserm-00995736

https://www.hal.inserm.fr/inserm-00995736

Submitted on 23 May 2014

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The potential role of HIV-specific CD38-/HLA-DR+

CD8+ T cells in viral suppressive activity and

cytotoxicity in HIV controllers

Stéphane Hua, Camille Lecuroux, Asier Saez-Cirion, Gianfranco Pancino,

Isabelle Girault, Martine Sinet, Olivier Lambotte, Alain Venet

To cite this version:

Stéphane Hua, Camille Lecuroux, Asier Saez-Cirion, Gianfranco Pancino, Isabelle Girault, et al..

The potential role of HIV-specific CD38-/HLA-DR+ CD8+ T cells in viral suppressive activity and

cytotoxicity in HIV controllers. BMC Infectious Diseases, BioMed Central, 2014, 14 (Suppl 2), pp.P64.

�inserm-00995736�

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P O S T E R P R E S E N T A T I O N

Open Access

The potential role of HIV-specific CD38-/HLA-DR+

CD8+ T cells in viral suppressive activity and

cytotoxicity in HIV controllers

Stéphane Hua

*

, Camille Lecuroux, Asier Saez-Cirion, Gianfranco Pancino, Isabelle Girault, Martine Sinet,

Olivier Lambotte, Alain Venet

From Abstracts from International Symposium HIV and Emerging Infectious Diseases 2014

Marseille, France. 21-23 May 2013

Introduction

In HIV-1 infection, some rare patients called HIV con-trollers (HICs) are capable to spontaneously control viral replication in vivo. Interestingly, HICs exhibit higher fre-quency of a particular activated phenotype CD38-HLA-DR+ HIV-specific CD8+ T cells. The aim of this study was to characterize this profile and evaluate its role in HICs.

Materials and methods

To investigate the functionality of the CD38-HLA-DR+ profile, we compared it with the classically activated phe-notype CD38+HLA-DR+ by evaluating several qualitative parameters: (1) activation measured by CD69, CD25, CD71, CD40 and Ki67 expression, (2) memory parameters measured by proliferation capacity, CD127 and Bcl-2 expression, cytokine production measured by IL-2 produc-tion and (3) cytotoxic activity. We also determined the mechanism responsible for this particular profile.

Results

CD38-HLA-DR+ cells exhibited a more resting profile than CD38+HLA-DR+ cells marked by a lower expression of several activation markers. Although they presented similar ex vivo profile especially concerning survival, IL-2 produc-tion, CD38-HLA-DR+ cells displayed significantly higher HIV-specific cytotoxic capacity after in vitro culture com-pared to CD38+HLA-DR+ cells (13% [7%-23%] vs. 7% [3%-11%], p=0.02). Furthermore only the frequency of CD38-HLA-DR+ HIV-specific CD8+ T cells correlated with the capacity of CD8+ T cells to inhibit viral replication ex vivo (r=0.32, p<0.0001). Moreover, the CD38-HLA-DR+

profile was preferentially displayed after activation by low doses of antigen. These results are in line with the enhanced expression of this profile in patients which exhi-bit high functional sensitivity (r=0.41, p=0.01).

Conclusions

Collectively, these data highlight the cytotoxic role of CD38-HLA-DR+ expressing HIV-specific CD8+ T cells in HICs and we provide insights into the mechanism of its induction. Induction of this type of protective cell subset could be an important goal in vaccine strategies.

Published: 23 May 2014

doi:10.1186/1471-2334-14-S2-P64

Cite this article as:Hua et al.: The potential role of HIV-specific CD38-/ HLA-DR+ CD8+ T cells in viral suppressive activity and cytotoxicity in HIV controllers. BMC Infectious Diseases 2014 14(Suppl 2):P64.

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Submit your manuscript at www.biomedcentral.com/submit INSERM U1012, Le Kremlin Bicêtre, France

Hua et al. BMC Infectious Diseases 2014, 14(Suppl 2):P64 http://www.biomedcentral.com/1471-2334/14/S2/P64

© 2014 Hua et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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