HAL Id: hal-02819490
https://hal.inrae.fr/hal-02819490
Submitted on 6 Jun 2020
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Prevalence of several human bacterial pathogens in French soils
Aude Locatelli, Lionel Ranjard, Samuel Dequiedt, Claudy Jolivet, Géraldine Depret, Alain Hartmann
To cite this version:
Aude Locatelli, Lionel Ranjard, Samuel Dequiedt, Claudy Jolivet, Géraldine Depret, et al.. Prevalence of several human bacterial pathogens in French soils. 13. International Symposium on Microbial Ecology, Aug 2010, Seattle, United States. 1 p., 2010. �hal-02819490�
PS.04.023 PREVALENCE OF SEVERAL HUMAN BACTERIAL PATHOGENS IN FRENCH SOILS Locatelli, A*1; Ranjard, L2; Dequiedt, S2; Jolivet, C3; Depret, G1; Hartmann, A1
1INRA/Université de Bourgogne, INRA UMR MSE, 17 rue Sully BP86510, DIJON, France; 2INRA/Université de Bourgogne, INRA UMR MSE Plateforme Genosol, 17 rue Sully BP86510, DIJON, France; 3Unité InfoSol, INRA, 2163 Avenue de la Pomme de Pin, Orléans, France
Soil harboring human bacterial pathogens may contribute to water and food contamination and thus pose a risk for human health. The emergence of these pathogens might be due to changes in agricultural practices (i.e.
application of "fecal" fertilizers, sewage sludge and animal manure). This study evaluated samples (n=1489) from the French soil monitoring network project (RMQS) including cultivated, pasture and forest soils, to evaluate the prevalence of five pathogenic bacteria and establish relationships between prevalence and soil properties and biotic (i.e. abundance and diversity of soil microbial communities) factors. Soils were evaluated for the presence of Escherichia coli, Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus and Salmonella enterica by quantitative PCR. Species-specific primers and probe were designed. The specificity of the prs gene for L. monocytogenes and the ecf gene for E. faecalis was validated using pure strains. Quantitative recovery and method detection limits were established in microcosm assays with soil inoculated with varying amounts of bacteria. The prs marker was specific for L. monocytogenes, and the detection limit was about 103 copies per gram of soil. Among the 935 soil DNA extract tested to date, only one was positive for L.
monocytogenes with a concentration of 105 copies of prs target per gram of soil. The ecf marker was validated for the detection of E. faecalis, the detection limit was about 104 copies of ecf target per gram of soil. E. faecalis was detected in 39% of the studied soils (range of detection from 104 to 107 per g). Despite the fact that L.
monocytogenes is thought to be able to persist in environment, it was infrequently detected in the French soils tested. Further analyses are underway to monitor levels of the three other bacterial species. Correlation analyses will be undertaken to determine which soil factors are related to the abundance of these bacterial pathogens.
