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A novel method for assessing adherent single-cell stiffness in tension: design and testing of a substrate-based live cell functional imaging device

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Academic year: 2021

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Fig. 1 (a) Geometry and (b) mesh of simplified  representa-tions of a cell on PDMS substrates, as modeled in the finite element analysis
Fig. 3 FEA of substrates and cells of different compliances undergoing 8% strain. While on the moderately stiff substrate (100 kPa) the cell is not able to influence substrate deformation pattern and simply follows the applied strain, the soft substrate
Fig. 6 PDMS surface strain calibration. (a) Substrate-to-substrate and (b) intra-substrate variations are shown
Fig. 8 (a – c) Maximum princi- princi-pal strain drop plot of SAOS-2 in three different conditions (Normal, Cytochalasin-D  treat-ment, Glutaraldehyde treatment) seeded on a functionalized PDMS substrate undergoing 8%

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