HAL Id: hal-02738266
https://hal.inrae.fr/hal-02738266
Submitted on 2 Jun 2020
HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci- entific research documents, whether they are pub- lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.
L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Construction and characterization of microbial consortia for keratin degradation
D. Kang, Samuel Jacquiod, J. Herschend, S.J. Sørensen
To cite this version:
D. Kang, Samuel Jacquiod, J. Herschend, S.J. Sørensen. Construction and characterization of micro- bial consortia for keratin degradation. FEMS 2017, Jul 2017, Valence, Spain. 2628 p., 2017, White Biotechnology. �hal-02738266�
FEMS7-3175
White Biotechnology
CONSTRUCTION AND CHARACTERIZATION OF MICROBIAL CONSORTIA FOR KERATIN DEGRADATION
D. Kang1, S. Jacquiod2, J. Herschend1, S.J. Sørensen1
1University of Copenhagen, Department of Biology, Copenhagen, Denmark
2INRA, UMR 1347 Agroécologie, Dijon, France
Backgrounds
Europe, especially Denmark, heavily relies on import of soy protein for feed in its animal production.
Keratins refer to a group of insoluble, tough and recalcitrant protein material generated in large quantities as a waste-product in commercial slaughterhouses, originating from bristles and hooves.
Utilization of microbial consortia can play a crucial role as natural biodegradation process for keratin.
It can be transformed into more valuable feedstock through the release of small peptides and amino acids by microbial degradation.
Objectives
Identify microbial communities for keratin degradation and construction of stable microbial consortia for industrial applications.
Methods
A soil-born microbial consortium was enriched on keratin as the sole carbon source in sequential batch cultivations at room temperature and characterized by application of next generation sequencing and specific keratin degradation assays.
Conclusions
During six enrichment cycles, the procedure selected for stable and efficient keratin degrading
microbial consortia, mainly constituted by members of bacteroidetes and proteobacteria phyla. For the sake of industrial applications, we used a dilution-based method to reduce the diversity and isolate key component strains involved in efficient keratin degradation, while excluding potential cheaters and ease of controllable outputs. The consortia were structurally stable with the co-existence of four major microbes, comprising aerobic bacterial genera Chryseobacterium, Stenotrophomonas,
Pseudochrobactrum, Acinetobacter. Residual substrates proportion is similar when comparing with using the microbial consortium without dilution and approximate 20% after five days. This work has potential applications for a vast range of areas including food and feed, fisheries, biotechnologies and agriculture.
