Comparative study of survival and growth in classical clear water and multi-trophic recirculating systems during Pseudoplatystoma puntifer larval rearing

(1)

HAL Id: hal-02144325

https://hal.archives-ouvertes.fr/hal-02144325

Submitted on 30 May 2019

HAL is a multi-disciplinary open access

archive for the deposit and dissemination of sci-

entific research documents, whether they are pub-

lished or not. The documents may come from

teaching and research institutions in France or

abroad, or from public or private research centers.

L’archive ouverte pluridisciplinaire HAL, est

destinée au dépôt et à la diffusion de documents

scientifiques de niveau recherche, publiés ou non,

émanant des établissements d’enseignement et de

recherche français ou étrangers, des laboratoires

publics ou privés.

Comparative study of survival and growth in classical

clear water and multi-trophic recirculating systems

during Pseudoplatystoma puntifer larval rearing

Jesus Nuñez, Sylvain Gilles, Cristian Fernández, Fabrice Duponchelle,

Jean-François Renno, Mearia Darias, Frank David, Jesús Núñez

To cite this version:

Jesus Nuñez, Sylvain Gilles, Cristian Fernández, Fabrice Duponchelle, Jean-François Renno, et al..

Comparative study of survival and growth in classical clear water and multi-trophic recirculating sys-

tems during Pseudoplatystoma puntifer larval rearing. RIIA - 4TH INTERNATIONAL CONFER-

ENCE COCHABAMBA - BOLIVIA, Sep 2014, COCHABAMBA, Bolivia. 1 (1), 2015, Proceedings

of the 4th International Conference of the ”Research Network on Amazonian Ichthyofauna” (RIIA)

September 30 - October 2, 2014 Cochabamba, Bolivia. �hal-02144325�

(2)

Comparative study of survival and growth in classical clear water and multi-trophic

recirculated systems during Pseudoplatystoma puntifer larval rearing

Jesús%Núñez

^1*

,%Sylvain%Gilles

¹

,

^%

Chris7an%Fernandez

²

,%Fabrice%Duponchelle

¹

,%JeanAFrançois%Renno

¹

%&%Maria%Darias

¹

%

%%

1

%BOREA%A%UMR%IRD%207,%Montpellier,%France%

2%

IIAP,%AQUAREC,%Iquitos,%Perú%

*%

EAmail:%[email protected]%

%

%%%%%%%%%%%

INTRODUCTION

Doncella, Pseudoplatystoma punctifer ex. fasciatum, artificial breeding relies on the adaptation of larvae to dry food (weaning) after a period of brine shrimp (Artemia nauplii) feeding. The main objective of the current research was the development of a breeding protocol that reduces the use of Artemia nauplii, the weaning period, the adaptation of the larvae to dry feeding and finally decrease mortality during this period. In parallel to the feeding protocol two rearing systems, a classic clear water recirculation system and an integrated multi-trophic aquaculture system, were tested at the IIAP’s field station of Quistococha (Peru) to compare survival and growth of sibling larvae over a 36 days period.

RESULTS

MATERIAL & METHODS

Larvae were fed live freshly hatched Artemia (3 replicates) from 3 to 15 days post fertilization (DPF) in both environments in 30 L tanks. Then they were weaned with 2 semi-humid inert diets (3 replicates), for an 11-days period (from 16 to 27 DPF) followed by a 8-days period of dry food (from 28 to 36 DPF). Samplings were performed at 15, 27 and 36 DPF. Feeding changes were made progressively over a 3-days period.

Total larvae counts and individual sizes were determined from digital photographs of the rearing tanks, subsequently processed using NIH IMAGE J freeware. Mean total length (TL) and survival, calculated for each replicate at each sampling time, were analyzed using one-way ANOVA and post-hoc tests to compare treatments.

CONCLUSIONS

These results indicated that it was possible to obtain juveniles of about 5 cm (TL) adapted to dry food consumption in 36 days.

Artemia has proved, in both systems, to be an effective feeding strategy for early larval phase until 15 DPF. Classical clear water system performed better from 3 to 15 DPF, then both systems are comparable in terms of survival, while growth was significantly higher in classical clear water system over the 36-days rearing period with the T2 feed.

0%

5%

10%

15%

20%

25%

30%

35%

40%

45%

50%

S_T1 C_T1 S_T2 C_T2

Longitud Total (mm)

Tipo de circuito & Tratamiento Crecimiento comparado a 36 DPF

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

S_T1 C_T1 S_T2 C_T2

Supervivencia (%)

Tipo de circuito & Tratamiento Supervivencia comparada a 36 DPF 0%

5%

10%

15%

20%

25%

30%

35%

40%

S_T1 C_T1 S_T2 C_T2

Longitud Total (mm)

0%

10%

20%

30%

40%

50%

60%

70%

S_T1 C_T1 S_T2 C_T2

Supervivencia (%)

Tipo de circuito & Tratamiento Supervivencia comparada a 27 DPF

0%

10%

20%

30%

40%

50%

60%

S_T1 C_T1 S_T2 C_T2

Supervivencia 15-36 DPF (%)

Tipo de circuito & Tratamiento Supervivencia total comparada de 15 a 36 DPF

3"15%DPF% 16"27%DPF% 28"36%DPF%

Artemia Wet Feeding Dry Feeding

SAMPLINGS

Figure 1. Summary of Experimental Protocol

Figure 2. Growth (Total Length) comparisons of P. Punctifer juveniles reared in classic recirculated circuit (C) and in multitrophic system (S) at 15, 27 and 36 DPF with two different feeds (T1 and T2)

Figure 3. Survival comparisons of P. Punctifer juveniles reared in classic recirculated circuit (C) and in multitrophic system (S) at 15, 27 and 36 DPF with two different feeds (T1 and T2)

Figure 4. Global survival comparison of P. Punctifer juveniles, fed Artemia nauplii for 12 days, reared in classic recirculated circuit (C) and in multitrophic system (S), weaned from 15 to 36 DPF with two different feeds (T1 and T2)

0%

2%

4%

6%

8%

10%

12%

14%

16%

18%

S_T1 C_T1 S_T2 C_T2

Longitud Total (mm)

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

S_T1 C_T1 S_T2 C_T2

Supervivencia (%)

Tipo de circuito & Tratamiento Supervivencia comparada a 15 DPF

Pseudoplatystoma-punc0fer-ex.%fasciatum-

3 DPF 15 DPF 27 DPF 36 DPF