Yo un g-m ale Yo un g-f em ale Old -m ale Old -fe ma le 0 .0 0 .5 1 .0 1 .5 S k i n m RNA le ve l o f G AD PH (fo ld ch an ge ) Yo un g-m ale Yo un g-f em ale Old -m ale Old -fe ma le 0 .0 0 .5 1 .0 1 .5 H e a r t m RNA le ve l o f G AD PH (fo ld ch an ge ) m RNA le ve l o f G AD PH (fo ld ch an ge ) Y-W T Y-K O O-W T O-K O 0 .0 0 .5 1 .0 1 .5 F e m a le - h e a r t Y-W T Y-K O O-W T O-K O 0 .0 0 .5 1 .0 1 .5 F e m a le - s k in m RNA le ve l o f G AD PH (fo ld ch an ge ) Y-W T Y-K O O-W T O-K O 0 .0 0 .5 1 .0 1 .5 M a le - h e a r t m RNA le ve l o f G AD PH (fo ld ch an ge ) Y-W T Y-K O O-W T O-K O 0 .0 0 .5 1 .0 1 .5 M a le - s k i n m RNA le ve l o f G AD PH (fo ld ch an ge ) A B C D E F
Supplement Figure 1 No significant age- and gender-associated difference in GAPDH mRNA levels. mRNA expression of GAPDH is analyzed by qRT-PCR in dorsal skin (A) and heart (B) of female/male young and old WT mice. mRNA expression of GAPDH is also analyzed by qRT-PCR in the female dorsal skin (C), male dorsal skin (D), female heart (E) and male heart (F) in the mice of Y-WT, Y-KO,
O-WT and O-KO. Y-WT: young wild type; Y-KO: young Arg-II-/-; O-WT: old wild type; O-KO: old
Arg-II-/-. The values shown are mean ± SD. n indicates the number of animals of each experimental
group.
n=8 n=8
n=8 n=8
