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Simultaneous Extraction and Determination of Preservatives and Antioxidants in Juice Samples by an Optimized Microextraction Method Using Central Composite Design and Validated …

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Simultaneous Extraction and Determination of Preservatives

and Antioxidants in Juice Samples by an Optimized

Microextraction Method Using Central Composite Design and

Validated …

Authors

Abderrahmane Tighrine, Edgar Pinto, Armindo Melo, Isabel MPLVO Ferreira, Marzouk Mamou, Youcef Amir

Publication date

2019/1/1

Journal

Journal of AOAC International

Volume 102 Issue 1 Pages 208-216 Publisher AOAC International Description

Background:

Food additives are widely used in industries. Overall, these additives have a beneficial role, but if their concentration exceeds certain limits, they may have an adverse effect on human health.

Objective:

This study outlines the determination of benzoic acid (BA), sorbic acid (SA), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) in juices using dispersive liquid– liquid microextraction and HPLC–diode-array detection.

Methods:

Different parameters that significantly affect the extraction efficiency were optimized. The disperser and extraction solvents were acetone and chloroform, respectively. The other parameters were selected and optimized using two-level (2k) factorial and central composite designs, respectively. A full method validation using an accuracy profile for the total measurement error was carried out.

Results:

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