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Tax ubiquitylation and SUMOylation control the
dynamic shuttling of Tax and NEMO between Ubc9
nuclear bodies and the centrosome
Youmna Kfoury, Niclas Setterblad, Marwan El-Sabban, Alessia Zamborlini,
Zeina Dassouki, Hiba El Hajj, Olivier Hermine, Claudine Pique, Hugues de
Thé, Ali Saïb, et al.
To cite this version:
Youmna Kfoury, Niclas Setterblad, Marwan El-Sabban, Alessia Zamborlini, Zeina Dassouki, et al.. Tax ubiquitylation and SUMOylation control the dynamic shuttling of Tax and NEMO between Ubc9 nuclear bodies and the centrosome. 15th International Conference on Human Retroviruses: HTLV and Related Viruses, Jun 2011, Leuven and Gembloux, Belgium. pp.A146, �10.1186/1742-4690-8-S1-A146�. �inserm-00663646�
MEETING ABSTRACT
Open Access
Tax ubiquitylation and SUMOylation control the
dynamic shuttling of Tax and NEMO between
Ubc9 nuclear bodies and the centrosome
Youmna Kfoury1, Niclas Setterblad2, Marwan El-Sabban3, Alessia Zamborlini4, Zeina Dassouki1, Hiba El Hajj1, Olivier Hermine5, Claudine Pique6, Hugues de Thé4, Ali Saïb4, Ali Bazarbachi1*
From 15th International Conference on Human Retroviruses: HTLV and Related Viruses Leuven and Gembloux, Belgium. 5-8 June 2011
The HTLV-I oncoprotein Tax is critical for T cell trans-formation, acting mainly through NEMO binding and subsequent NF-B activation. Tax localizes to Tax nuclear bodies and to the centrosome and is subjected to ubiquitylation and SUMOylation that are both neces-sary for complete transcriptional activation. By using the photoconvertible fluorophore Dendra-2 coupled with live video confocal microscopy, we show for the first time that the same Tax molecule shuttles among Tax nuclear bodies and between these nuclear bodies and the centrosome depending on its post-translational modifications. Ubiquitylation targets Tax to nuclear bodies to which NEMO is recruited and subsequently SUMOylated. We also demonstrate that Tax nuclear bodies contain the SUMOylation machinery including SUMO and the SUMO conjugating enzyme Ubc9, strongly suggesting that these nuclear bodies represent sites of active SUMOylation. Finally, both ubiquitylation and SUMOylation of Tax control NEMO targeting to the centrosome. Altogether, we are proposing a model where both ubiquitylation and SUMOylation of Tax control the shuttling of Tax and NEMO between the cytoplasmic and nuclear compartments.
Author details
1Department of Internal Medicine, American University of Beirut, Beirut,
Lebanon.2Imagery Department of the Institut Universitaire d’Hématologie IFR105, Paris, France.3Department of Human Morphology, American
University of Beirut, Beirut, Lebanon.4UMR 7212 CNRS, U944 Inserm,
Laboratoire Associé au Comité de Paris de la Ligue contre le Cancer, Paris, France.5CNRS UMR 8603 and Department of Hematology, Necker Hospital,
Paris, France.6INSERM U1016, CNRS UMR 8104, Université Paris Descartes,
Institut Cochin, Department of cell Biology and Host-Pathogens Interactions, Paris, France.
Published: 6 June 2011
doi:10.1186/1742-4690-8-S1-A146
Cite this article as: Kfoury et al.: Tax ubiquitylation and SUMOylation control the dynamic shuttling of Tax and NEMO between Ubc9 nuclear bodies and the centrosome. Retrovirology 2011 8(Suppl 1):A146.
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* Correspondence: bazarbac@aub.edu.lb
1
Department of Internal Medicine, American University of Beirut, Beirut, Lebanon
Full list of author information is available at the end of the article Kfoury et al. Retrovirology 2011,8(Suppl 1):A146
http://www.retrovirology.com/content/8/S1/A146
© 2011 Kfoury et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
