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Human germinal center B cells differ from naive and memory B cells by their aggregated MHC class II‐rich compartments lacking HLA‐DO

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Academic year: 2021

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Fig. 3. Dual ligation of CD40 and BCR in naive B cells down- down-regulates HLA-DO. Puri®ed naive B cells were cultured for 6 days with irradiated L cells either transfected with CD32 alone, to maintain the naive phenotype, or double-transfected with CD40L
Fig. 6. Naive and GC B cells internalize BCR±anti-BCR complexes within MIIC. Puri®ed naive (upper panel) and GC (lower panel) B cells were incubated at 4°C with anti-k and anti-l antibodies, and then with Texas Red-conjugated secondary antibody as surrogat

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