PLATINUM DISTRIBUTION IN INSECT TESTIS AFTER CIS-DIAMMINEDICHLOROPLATINUM TREATMENT, AN EELS STUDY IN THE HOUSE CRICKET, ACHETA DOMESTICUS

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Submitted on 1 Jan 1984

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PLATINUM DISTRIBUTION IN INSECT TESTIS AFTER CIS-DIAMMINEDICHLOROPLATINUM TREATMENT, AN EELS STUDY IN THE HOUSE

CRICKET, ACHETA DOMESTICUS

F. Hawkes

To cite this version:

F. Hawkes. PLATINUM DISTRIBUTION IN INSECT TESTIS AFTER CIS- DIAMMINEDICHLOROPLATINUM TREATMENT, AN EELS STUDY IN THE HOUSE CRICKET, ACHETA DOMESTICUS. Journal de Physique Colloques, 1984, 45 (C2), pp.C2- 589-C2-590. �10.1051/jphyscol:19842136�. �jpa-00223806�

JOURNAL DE PHYSIQUE

Colloque C2, suppl6ment au n02, Tome 45, f6vrier 1984 page C2-589

PLATINUM D I S T R I B U T I O N I N INSECT T E S T I S AFTER

CIS-DIAMMINEDICHLOROPLATINUM TREATMENT, AN EELS STUDY I N THE HOUSE CRICKET, ACHETA DOMESTICUS

F . Hawkes

Uboratoire d1@tique Etectronique du CNRS

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Resume : Apres t r a i t e m e n t avec l e d - d i a m m i n e d i c h l o r o p l a t i n u m (11), on trouve du p l a - m a n s l e chromatine e t l e s chromosomes, dans l e n u c l e o l e e t dans l e s lysosomes.

A b s t r a c t : A f t e r treatment w i t h cis-diamminedichloroplatinum (11) , p l a t i n u m i s found i n chromatin and chromosomes, i n n u c l e o l i and i n lysosomes.

C i s p l a t i n o r cin-diamminedichloroplatinum (11) , the f i r s t metal c o o r d i n a t i o n complex t o be introduced i n cancer chemotherapy, i s used c l i n i c a l l y f o r t h e treatment o f head and neck cancer, o v a r i a n carcinoma, t e s t i c u l a r teratoma and o t h e r cancers. T h i s promp- t e d us t o study i t s e f f e c t s on meiosis and spermiogenesis.

With the a i d o f EELS, we detected p l a t i n u m i n chromatin, chromosomes, n u c l e o l i and lysosomes.

CHROMOSOME (Metaphasel

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&h 20 1-14 06 cispla.tin.

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' ~ s s o c i 6 5 I'Universit6 Paul Sabatier

Article published online by EDP Sciences and available at http://dx.doi.org/10.1051/jphyscol:19842136

C2-590 JOURNAL DE PHYSIQUE

The house c r i c k e t s were i n j e c t e d i n t r a - a b d o m i n a l l y w i t h 10 p1 o f an aqueous s o l u t i o n o f c i s p l a t i n . The doses were 5 pg, 10 pg and 20 ug per i n s e c t . The c r i c k e t s i n j e c t e d w i t h a dose o f 20 ug were a l l dead (24) a f t e r 7 days. A l l survived f o r 4-6 h. We, therefore, examined o t h e r animals i n j e c t e d w i t h 20 pg, t h e treatments l a s t i n g 2 h 30 and 4 h ; we obtained c l e a r peaks. C r i c k e t s i n j e c t e d w i t h 10 pg o f c i s p l a t i n were i n poor c o n d i t i o n a f t e r 3 days, t h e m a j o r i t y having died. With a dose o f 5 ug per i n s e c t and a d u r a t i o n o f treatment o f 1 h, 2 h, 3 h o r 4 h, t h e c r i c k e t s were i n apparent good h e a l t h and very l i v e l y ; nevertheless, as seen i n TEM, t h e r e was a marked a c t i o n on chromatin, chromosomes and n u c l e o l i .

The analyses were performed on m a t e r i a l f i x e d i n 2.5 % b u f f e r e d glutaraldehyde, w i t h - o u t osmium treatment, and embedded i n Epon. The sections were 200 nm t h i c k and s t a i - ned w i t h uranyl acetate. The energy l o s s spectrum, corresponding t o a s e l e c t e d area 100 nm i n diameter, was obtained w i t h an 0 - f i l t e r connected t o the Toulouse 1.2 MV microscope and analysed w i t h t h e a i d o f a Tracor TN-11 u n i t . We worked a t 1 MV. The M45 l i n e o f p l a t i n u m was detected a t 2200 eV i n chromatin, chromosomes, n u c l e o l i and lysosomes. The EELS study was i n good agreement w i t h our r e s u l t s i n TEM. A l l i n a l l , the a c t i o n o f c i s p l a t i n i s very s i m i l a r t o t h a t o f the o t h e r a l k y l a t i n g agents we studied.

This EELS study would n o t have been p o s s i b l e w i t h o u t the h e l p o f Madame Kihn w i t h t h e Tracor system. We thank her most warmly.