A survey for citrus psorosis- associated virus in a citrus collection of Southern Italy Djelouah K., D' Onghia A.M. in

Djelouah K., D' Onghia A.M.

in

Martelli G.P. (ed.), D' Onghia A.M. (ed.).

Proceedings of the Mediterranean network on certification of citrus. 1995-1997 Bari : CIHEAM

Options Méditerranéennes : Série B. Etudes et Recherches; n. 21 1998

pages 107-111

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--- D jelouah K., D 'Onghia A.M. A su rvey for citru s psorosis-associated viru s in a citru s collection of Sou th ern Italy. In : Martelli G.P. (ed.), D 'Onghia A.M. (ed.). Proceedings of the Mediterranean network on certification of citrus. 1995-1997. Bari : CIHEAM, 1998. p. 107-111 (Options Méditerranéennes : Série B. Etudes et Recherches; n. 21)

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Dipartimento di delle dalle e Centro di Studio del sui Virus e le Virosi delle Colture

Università degli Studi di Bari Bari

A.

Agronomic Bari

-

collection held at the of Valenzano at the

di delle dalle of -

was assessed using a kit at the di Applicata (1FA)-

et al., 1997). was in most of the

of a local clementine

CV. ‘Fedele’. in detecting the

of of this

words: diagnosis,

- La présence du virus associé à la psorose des agrumes été évaluée dans une collection internationale d‘agrumes, soumis au préalable à des essais d‘indexage pour la psorose, à Agronomique de Valenzano (Bari) et au Dipartimento di

delle dalle de de Bari, cette un

kit a été utilisé> produit par di Fitovirologia Applicata Turin, (Garcia et al., 1997)- s’est avérée être largement répandue chez la plupart des variétés d‘agrumes. a confirmé les résultats obtenus par indexage, à d‘un cultivar local de clémentinier, “Fedele”. deux échantillons de a été plus sensible que rindexage dans la détection du virus. La preuve de la fiabilité et de la sensibilité du test représente un résultat important en vue d‘un indexage sérologique de routine de ce virus et pour 1’ éventuelle certification.

agrumes, diagnostic,

Options Méditerranéennes, Série B /no21, - Proceedings of the Mediterranean Network on Certification of Citrus

108 Djelouah, A. D’Onghia

The is in the (Sicily, Apulia,

and Campania). Apulia cultivated along the coast and to a extent

in and Salento, 10,000 ha.

The most species the Navel (‘Naveline’, ‘Washington Navel’, ‘Newhall’), di Ciaculli’), clementines (‘Common’

clementine and the local ecotypes ‘Fedele’, di Satsuma.

of the diseases have in this is the

most 1998) as in 1993).

is a complex of diseases which

et al., 1991, 1993), being often

symptomless in most The putative causal agent is a filamentous belonging to a

new taxon called et a1.,1993), et al., 1994; et al.,

1996).

the classical biological indexing, been successfully used

kit the et al., 1997).

The use of this in is the analysis of plants of

and held in the collection of the of the

biologically indexed V. Savino, Ten

isolates positive of concave gum isolates a

possible to included in the test.

A total of 177 samples taken in 1996 all plants in collection

held the mainly coming Apulia

and Lebanon, Spain 1). All of

indexed to dweet seedlings positive

negative 1993). tested

et al., 1997). Wells of flexible plates (Falcon 391 1)

coated with at 1.4 pg/ml in plus 0.01% sodium azide, then

incubated about 2 h at 37°C. collected young leaf

samples and ten times diluted in saline with 0,05% tween 20 containing 2% and incubated at 4” C. Conjugated alkaline phosphatase, diluted 1/1000 in was added and incubated for 2h at 37°C. The (lmg/ml of

phosphate) was then added, and the The on

A survey for citrus psorosis-associated virus in a citrus collection of Southern 109

the 2 wells loaded with each positive the values 3 example, isolate

value 15 Ten

of of

gum (CG) isolates of C.N.

evaluate a possible in mixed

infection. The of tests, in 1996, was to check the obtained in the same plants.

and

The (Table 1) indicate that is in most of the

All the plants that indexed positive positive in

sample of a local clementine CV. Fedele. These suggest that the test of the

Two samples of CV. Navelina, which indexed as negative in the biological test, found to be in both the test, suggesting that in these cases the

sensitive than indexing in detecting the

All the plants of CV. et al.,

oak leaf symptoms in the field and index tests, be in No

local concave gum that was negative the

the USA so it is assumed that the level of mixed infection with the two agents is in the local tested.

The the high

of the assay in the

symptoms and the of to These

in the and an additional aid to

The with-two samples that had indexed as healthy could indicate that these

that might in the context of in

l

110 Djelouah, A. D'Onghia

Table

-

Citrus varieties Tested

29 4 15

6 4

11 5 11

20 5 6 4 5

14

4 3 3 2 3

1

2 2 15

1 2

Positive by indexing

11 2

1 3 O

1 2 O

2 4 2 2 O

8

1 O O O 2

1

O 2 O O

,i,

,

4

__

, -. _,.

Positive by Origin

Lebanon

Lebanon

Lebanon

[taly

. . , . . . - . . ., '. . ,. _{, ,} ~ ^' * . . . . . ^I. . *-= " . ^{, . _} .

A survey for citrus psorosis-associated virus in a citrus collection of Southern 111

Lee, G.A. and J.V. (1991). of

Conf. pp. 386-390.

Lee, and G.A. (1993).

plant associated with 12 Conf. pp.

428-430.

and Savino, V. (1992). casi di concavità gommose su Navelina in Fitopatol., 3, pp. 39-41.

and diseases in with special to Apulia. In

the on Certification Citrus 1995-1997, Options

Series B 21, publications, pp. 65-71.

E., O. and (1994). The closely

have of J . Gen. Virol, 75, pp. 3585-3590.

Sanchez E., N., Luisoni, E., and

O. (1997). of

46(6), pp. 830-836.

E. and O. (1996). of

implications diagnosis and taxonomy. 13th Conf.

China, pp. 189 -197.

C.N. and Calavan, E.C. (1965). studies with of concave gum and

3rd Col$ pp. 154 -161.

C.N. (1993). - a pp. 139 -154.