Supplemental Figure 2. PCA of NMR measurements and metabolite quantification for samples grouped by treatment. Difference in metabolic profiles of Norm and Alsen wheat when treated with Fg is apparent with major difference in DON+(9F1 and Gz3639) and smaller but still observable difference in the DON-Gzt40 strain. Close agreement in the sample separation in PCA results when analyzing spectra and quantified data is apparent. Spectra have been
normalized using NMR reference trimethylsilylpropionate and relative concentrations of metabolites obtained using multivariate linear regression have been normalized to norm 1. Methanol peak has been removed from analysis.