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Interdomain Flexibility within NADPH Oxidase Suggested by SANS Using LMNG Stealth Carrier

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FIGURE 1 Organization of NOX enzymes and related complexes. (A) Organization of neutrophilic NOX complex composed of the membrane catalytic subunit NOX2, p22 phox , and the cytosolic factors p47 phox , p67 phox , p40 phox , and Rac
FIGURE 2 Optimization of SpNOX thermostability in detergent. (A) Normalized thermal unfolding curves of His-SpNOX (0.8 mg mL 1 ) in the presence of 300 mM NaCl for several detergents: DDM (orange), DMNG (gray), and LMNG (blue)
FIGURE 3 Optimization of pH and salt concentration to promote SpNOX thermostability. (A) Thermal unfolding curves of SpNOX (0.8 mg mL 1 ) in buffers containing 300 mM NaCl with various pHs: (dark blue) pH 9, (light blue) pH 8, (orange) pH 7, (green) pH 6,
FIGURE 5 SEC-SANS experiment. (A) Preparative SEC profile of SpNOX, eluted on a HiLoad 16/600 Superdex 200 column, in 50 mM Tris-HCl (pH 7), 300 mM NaCl, 10 m M FAD, and 0.025 mM LMNG
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