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Antisense protein of HTLV-2 (APH-2) associates with PML nuclear bodies: molecular determinants and functional implications

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HAL Id: inserm-00924975

https://www.hal.inserm.fr/inserm-00924975

Submitted on 7 Jan 2014

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Antisense protein of HTLV-2 (APH-2) associates with

PML nuclear bodies: molecular determinants and

functional implications

Chloé Journo, Jocelyn Turpin, Estelle Douceron, Anaïs Oliva, Renaud

Mahieux

To cite this version:

Chloé Journo, Jocelyn Turpin, Estelle Douceron, Anaïs Oliva, Renaud Mahieux. Antisense protein

of HTLV-2 (APH-2) associates with PML nuclear bodies: molecular determinants and functional

implications. 16th International Conference on Human Retroviruses: HTLV and Related Viruses, Jun

2013, Monreal, Canada. pp.P100, �10.1186/1742-4690-11-S1-P100�. �inserm-00924975�

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P O S T E R P R E S E N T A T I O N

Open Access

Antisense protein of HTLV-2 (APH-2) associates

with PML nuclear bodies: molecular

determinants and functional implications

Chloé Journo

*

, Jocelyn Turpin, Estelle Douceron, Anaïs Oliva, Renaud Mahieux

From

16th International Conference on Human Retroviruses: HTLV and Related Viruses

Montreal, Canada. 26-30 June 2013

Antisens Protein of HTLV-2 (APH-2) was described in 2009. APH-2 mRNA is expressed in vivo in most HTLV-2 carriers. In recent years, several laboratories have searched for similarities and/or differences between APH-2 and the antisens protein of HTLV-1, HBZ. Similarly to HBZ, APH-2 negatively regulates HTLV-2 transcription. How-ever, it does not promote cell proliferation. In vivo, APH-2 localizes in discrete nuclear domains distinct from nucleoli. We therefore characterized APH-2 subcellular localization, in order to decipher the determinants of such localization and to correlate it or not with APH-2 func-tions. We first identify APH-2-containing nuclear domains as PML nuclear bodies (PML-NB). PML-NB are modula-tors of a number of cellular processes ranging from tran-scription regulation to cell proliferation and death. We show that both an in silico-identified nuclear localization signal and the carboxy-terminal LXXLL motif contribute to APH-2 targeting to PML-NB. Covalent modification of APH-2 by SUMO-1 and non-covalent interaction between APH-2 and SUMO-1-modified cellular partners have also been investigated as mechanisms of APH-2 targeting to PML-NB. Our results further demonstrate that APH-2 association with PML-NB is critical for its ability to inhibit viral transcription. This association also leads to a striking decrease in APH-2 stability, suggesting that APH-2 might be active but also targeted to degradation in PML-NB. Finally, we show that APH-2 localization in PML-NB leads to PML-NB clustering and correlates with a decrease in cell proliferation. Altogether, our study sheds new light on the links between the subcellular localization of APH-2 and its cellular functions.

Published: 7 January 2014

doi:10.1186/1742-4690-11-S1-P100

Cite this article as:Journo et al.: Antisense protein of HTLV-2 (APH-2) associates with PML nuclear bodies: molecular determinants and functional implications. Retrovirology 2014 11(Suppl 1):P100.

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* Correspondence: chloe.journo@ens-lyon.fr

Oncogenèse Rétrovirale, Equipe Labellisée Ligue Nationale Contre le Cancer, CIRI, INSERM U1111-CNRS UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, LabEx ECOFECT, Lyon, France

Journo et al. Retrovirology 2014, 11(Suppl 1):P100 http://www.retrovirology.com/content/11/S1/P100

© 2014 Journo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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