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Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells

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Academic year: 2021

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Figure 1 Assessment of the TLDA reproducibility. The TLDA reproducibility was assessed by testing, from one RNA sample, two separate qPCR amplifications from A- the same RT and pre-amplification product (RT1-d1 vs RT1-d2: Bland-Altman) and B- separate RT a
Figure 2 Impact of the RNA quantity on miRNA TLDA profiles.
Figure 3 Comparison of miRNAs expression profiles from human PBMCs and serum. Human PBMCs (1×10 6 cells) and serum (300 μ L) samples were extracted by Macherey-Nagel (MN) and Qiagen (Q) kits
Figure 4 Assessment of bias in RNA isolation using Macherey-Nagel kit from 300 or 600 μ L serum
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