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Plant-produced chimeric VHH-sIgA against enterohemorrhagic E. coli intimin shows cross-serotype inhibition of bacterial adhesion to epithelial cells

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FIGURE 1 | Design and production of individual subunits required for chimeric secretory IgA assembly
FIGURE 2 | Optimization of the accumulation levels of V H H-sIgA subunits after transient transformation of N
FIGURE 3 | V H H3-sIgA subunits physically associate with one another. N. benthamiana leaf tissue co-infiltrated with all V H H3-sIgA subunits was collected at 6 dpi, and recombinant proteins were immunoprecipitated with an anti-c-Myc antibody
FIGURE 4 | V H H9-sIgA subunits assemble in vivo over time. N. benthamiana leaves were co-infiltrated with a mixture of the indicated Agrobacterium strains, and leaf samples were collected from 4 to 12 dpi
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