Molecular characterization and prospective isolation of human fetal cochlear hair cell progenitors

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Molecular characterization and prospective isolation of human fetal cochlear hair cell progenitors

ROCCIO, Marta, et al.

Abstract

Sensory hair cells located in the organ of Corti are essential for cochlear mechanosensation.

Their loss is irreversible in humans resulting in permanent hearing loss. The development of therapeutic interventions for hearing loss requires fundamental knowledge about similarities and potential differences between animal models and human development as well as the establishment of human cell based-assays. Here we analyze gene and protein expression of the developing human inner ear in a temporal window spanning from week 8 to 12 post conception, when cochlear hair cells become specified. Utilizing surface markers for the cochlear prosensory domain, namely EPCAM and CD271, we purify postmitotic hair cell progenitors that, when placed in culture in three-dimensional organoids, regain proliferative potential and eventually differentiate to hair cell-like cells in vitro. These results provide a foundation for comparative studies with otic cells generated from human pluripotent stem cells and for establishing novel platforms for drug validation.

ROCCIO, Marta, et al . Molecular characterization and prospective isolation of human fetal cochlear hair cell progenitors. Nature Communications , 2018, vol. 9, no. 1, p. 4027

PMID : 30279445

DOI : 10.1038/s41467-018-06334-7

Available at:

http://archive-ouverte.unige.ch/unige:121806

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File Name: Supplementary Data 1

Description: Gene expression data from the 96.96 gene array.

Indicated are the mean Ct values obtained in the assay from 3 technical replicates, corrected for the expression levels of beta-actin (ACTB) and GAPDH (ΔCt). Columns are ranked with highly expressed genes on top.

Two cochlear duct (CD) samples, two utricle (UT) samples and two spiral ganglion (SG)

samples from the same donors at week 8.3 (W8.3) and week 11.1 (W11.1) are analyzed. For the spiral ganglion, two additional samples are analyzed: SG_W9 and SG_W11.8

File Name: Supplementary Data 2

Description: List of primers used in the study for the 96.96 gene array.

Gene symbol, plate and forward (FP) and reverse (RP) primers are indicated.

Description of Additional Supplementary Files

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