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Point-spread function retrieval for fluorescence microscopy

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Fig. 1: Schematic showing the imaging of a fluorescent microsphere in a medium of refractive index n s (with n s 6= n i ).
Fig. 2: Lateral (top) and axial (bottom) sections of the (a) simu- simu-lated band-limited microsphere, (b) the theoretically modeled PSF (5) at a depth of 10µm, and the corresponding (c) blurred-noisy (γ = 100) simulation of the object as imaged by a CLSM
Fig. 3: Axial profiles of a calculated PSF, the blurred microsphere and the observed microsphere

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