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Determination of methionine and selenomethionine in selenium-enriched yeast by species-specific isotope dilution with liquid chromatography-mass spectrometry and inductively coupled plasma mass spectrometry detection

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Academic year: 2021

Partager "Determination of methionine and selenomethionine in selenium-enriched yeast by species-specific isotope dilution with liquid chromatography-mass spectrometry and inductively coupled plasma mass spectrometry detection"

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Table 1. Experimental Conditions ICPMS Operating Conditions nebulizer gas flow 0.7 L/min
Table 2. Isotopic Distributions of Natural and Enriched Forms of Met and SeMet Used in Eq 1 for LC
Figure 2. Anion-exchange ICPMS chromatograms of (a) Se standards. Peaks: 1, seleno- DL -cystine; 2, seleno- DL -methionine; 3, selenite; 4, selenate; 5, selenoadenosylhomocysteine, (b) Selenized yeast digest on AE ICPMS with the internal standard addition
Figure 3. AE ICPMS chromatograms of (a) natural abundance standard and (b) selenized yeast digest spiked with 74 Se-enriched SeMet.

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