• Aucun résultat trouvé

OA031-02. Regulatory T cells inhibit CD8 T cell proliferation in HIV-1 infection through CD39/adenosine pathway

N/A
N/A
Protected

Academic year: 2021

Partager "OA031-02. Regulatory T cells inhibit CD8 T cell proliferation in HIV-1 infection through CD39/adenosine pathway"

Copied!
2
0
0

Texte intégral

(1)

HAL Id: inserm-00663922

https://www.hal.inserm.fr/inserm-00663922

Submitted on 27 Jan 2012

HAL is a multi-disciplinary open access

archive for the deposit and dissemination of

sci-entific research documents, whether they are

pub-lished or not. The documents may come from

teaching and research institutions in France or

abroad, or from public or private research centers.

L’archive ouverte pluridisciplinaire HAL, est

destinée au dépôt et à la diffusion de documents

scientifiques de niveau recherche, publiés ou non,

émanant des établissements d’enseignement et de

recherche français ou étrangers, des laboratoires

publics ou privés.

OA031-02. Regulatory T cells inhibit CD8 T cell

proliferation in HIV-1 infection through

CD39/adenosine pathway

Maria Nikolova, Matthieu Carrière, Jean-Daniel Lelievre, Maria Muhtarova,

Armand Bensussan, Yves Lévy

To cite this version:

Maria Nikolova, Matthieu Carrière, Jean-Daniel Lelievre, Maria Muhtarova, Armand Bensussan,

et al.. OA031-02. Regulatory T cells inhibit CD8 T cell proliferation in HIV-1 infection through

CD39/adenosine pathway. AIDS Vaccine 2009, Oct 2009, Paris, France. pp.O20,

�10.1186/1742-4690-6-S3-O20�. �inserm-00663922�

(2)

BioMed Central

Page 1 of 1

(page number not for citation purposes)

Retrovirology

Open Access

Oral presentation

OA031-02. Regulatory T cells inhibit CD8 T cell proliferation in

HIV-1 infection through CD39/adenosine pathway

M Nikolova

1

, M Carriere

2

, J Lelievre

3

, M Muhtarova

1

, A Bensussan

2

and

Y Lévy*

3

Address: 1National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria, 2INSERM, Unité U955, Créteil, France and 3Immunologie clinique, AP-HP, Groupe Henri-Mondor Albert-Chenevier; Université Paris 12, Créteil, France

* Corresponding author

Background

Generation of CD8 T cell responses following viral infec-tion or vaccinainfec-tion is indispensable for infecinfec-tion control. Regulatory CD4+ CD25+ T cells (Treg) are a key factor for the inefficiency of CD8 responses in viral persistence. The mechanisms of this suppression are not elucidated. Treg constitutively express the ectonucleotidase CD39, that generates immunosuppressive adenosine in the sites of immune activation.

Methods

HIV+ patients either treated or not with antivirals (CD4 >350 cells/mkl, n = 47) were studied in parallel with HIV-controls (n = 25). CD8 and Treg cells were purified by magnetic beads separation. Anti-CD3 stimulated CD8 T cell proliferation was assessed on CFSE-stained CD8 T cells cultured in the presence of either: i) Treg preincu-bated with anti-CD39 blocking mAb or isotype control; ii) adenosine analogue CGS 21680. Expression of the adeno-sine A2A receptor (A2AR) was quantified by RT-PCR.

Results

Treg – associated CD39 expression was significantly increased in HIV+ patients as compared to HIV- controls (mean CD39+ Treg %2.1 vs. 0.98 and mean CD39 MFI 1011 vs. 606, P < 0.01 for both). In the presence of Treg, proliferation of HIV+ CD8 T cells was significantly inhib-ited (mean inhibition 56% vs. 22.5% for HIV- controls; P < 0.01), while the inhibition decreased to an average of

12.3% (P < 0.01) in the presence of anti-CD39 blocking mAb. HIV+ CD8 T cells were characterized by an elevated expression of A2AR. The inhibitory effect of Treg on HIV+ CD8 T cell proliferation was reproduced by adenosine agonist. Finally, the percentage of Treg CD39+ was corre-lated with plasma HIV RNA values in HIV+ treatment-naive patients and inversely with CD4 AC.

Conclusion

Treg CD39-adenosinergic axis is involved in the progres-sion of chronic HIV-1 infection and Treg-mediated inhibi-tion of CD8 T cell proliferainhibi-tion. Modulainhibi-tion of Treg CD39+ function might be an attractive approach for enhancing CD8 T lymphocyte responses.

from AIDS Vaccine 2009

Paris, France. 19–22 October 2009 Published: 22 October 2009

Retrovirology 2009, 6(Suppl 3):O20 doi:10.1186/1742-4690-6-S3-O20

<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>

This abstract is available from: http://www.retrovirology.com/content/6/S3/O20 © 2009 Nikolova et al; licensee BioMed Central Ltd.

Références

Documents relatifs

 ISSEA, Institut Sous-régional de Statistique et d'Économie Appliquée de Yaoundé  ENSAE, École Nationale de la Statistique et de l'Analyse Économique de Dakar  ENEAM,

Au final, ce ‘milieu’, reçu en héritage par Laurent Mauvignier vaut bien, sans déni de sa part, comme une mise en abyme de la société toute entière, régie par

Vu le manque de preuves et la vulnérabilité de cette population, le CBIP rappelle que les statines doivent être prescrites avec prudence et que la prescription ou la déprescription

Isolated human testicular germ cells express several alternative HIV receptors and support the attachment of HIV-1 R5 and X4 strains.. Heparan sulfate proteoglycans and mannose

L’accès à ce site Web et l’utilisation de son contenu sont assujettis aux conditions présentées dans le site LISEZ CES CONDITIONS ATTENTIVEMENT AVANT D’UTILISER CE SITE WEB.

Moreover, compared to standard detectors, the 4 sub-band energy detector reduces the false alarm rate from 44% to 36%, increases the detection rate from 66% to 79% and augments

Louis Wicht du Locle Après avoir été près d'un quart de siècle président d'une société fribourgeoise du dehors, j'ai senti le besoin de me rendre dans ce beau village de

The objective of the packing is to minimize the total number of bins into which the descendants of an internal node are packed, summed over all internal nodes.. We investigate