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WC-1 and the proximal GATA sequence mediate a cis-/trans-acting repressive regulation of light-dependent gene transcription in the dark

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Academic year: 2021

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Figure S1. (A) WC-1 was detected on extracts (input used for IP in Figure 1C) obtained from wc-1 null mutant (negative control), wt and wmn mutant in the dark and after illumination. Arrows indicate different sizes for WC-1 in Wt and wmn mutant. (B) WC-2 was detected in wt and wmn samples.

Figure S2. Phenotype analysis related to Figure 3B. Replica experiments performed on WT WC-1 Myc and WC-1 Myc ZnF ∆ mutant following the protocol described in Figure 3B.

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Figure S3. (A) Row data representing ChIP replicas used for quantification in Figure 4D (n1, n2 and n3 showed in (B). Replica n2 is shown in the main figure as representative example. (B) Comparison between representative ChIP analyses performed in wt WC-1 Myc and ZnF ∆ mutant.

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Figure S2. Phenotype analysis related to Figure 3B. Replica experiments performed on WT WC-1 Myc  and WC-1 Myc ZnF ∆ mutant following the protocol described in Figure 3B
Figure S3. (A) Row data representing ChIP replicas used for quantification in Figure 4D (n1, n2 and  n3 showed in (B)

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