Immune response to functionalized mesoporous silica nanoparticles for targeted drug delivery

A

B

0 200 400 600 In te rle uk in -1 s ec re tio n [p g/ m l] 0 200 400 600 800 1000 In te rle uk in -6 s ec re tio n [p g/ m l] Unstim. Recomb. cytokine 0.1 1.0 10 0.1 1.0 10 0 500 1000 1500 In te rle uk in -1 2p 70 s ec re tio n [p g/ m l] MSN-NH2 g/ml] [ MSN (bare) [g/ml] 0.1 1 O pt ic al d en si ty a t 4 50 n m [a rb itr ar y un its ] No MSN MSN (bare) MSN-NH₂ Interleukin-1 0.1 1 O pt ic al d en si ty a t 4 50 n m [a rb itr ar y un its ] Interleukin-6 10 100 1000 0.1 1

Set cytokine concentration [ g/ml]

O pt ic al d en si ty a t 4 50 n m [a rb itr ar y un its ] Interleukin-12p70

Supplementary Figure S1: Cytokines are not adsorbed to the cationic surface of MSN-NH2.

(A) MSN-NH2 and unfunctionalized (bare) MSN were incubated with defined concentrations of recombinant cytokines. After 18 h, the concentration of the indicated cytokines in the culture medium was analyzed by

ELISA. (B) Complete splenocytes were incubated for 18 h with different concentrations of MSN-NH₂ or bare

MSN. The amounts of the pro-inflammatory cytokines IL-1β, IL 6 and IL 12p70 in the cell culture supernatant were determined by ELISA. Data give the mean value of triplicate samples ± s.e.m. All results are represen-tative of two independent experimenta.

Electronic Supplementary Material (ESI) for Nanoscale.

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