Genetic predisposition and environmental factors associated with the development of atopic dermatitis in infancy: a prospective birth cohort study
Journal : European Journal of Pediatrics
Caroline Gallay1,4, Patrick Meylan1,4, Sophie Mermoud1, Alexandre Johannsen1, Caroline Lang1, Carlo Rivolta2,3 and Stéphanie Christen-Zaech1
1Pediatric Dermatology Unit, Departments of Pediatrics and Dermatology, Lausanne University Hospital, Switzerland
2Department of Computational Biology, Unit of Medical Genetics, University of Lausanne, Switzerland
3Department of Genetics and Genome Biology, University of Leicester, United Kingdom
4these authors contributed equally to this work
Corresponding author
Dr. Stephanie Christen-Zaech, PD, MSCI Hôpital de Beaumont
Avenue de Beaumont 29 CHUV – 1011 Lausanne Tel. + 41 79 556 03 05 Fax: + 41 21 314 03 92 stephanie.christen@chuv.ch
Supplementary Material : 5 Questionnaires
Anamnestic questionnaire Number of the clinical trial : CHILD
1. What is the sex of the child? !! M
!! F 2. Difference from expected weight (g)? !! ≤-500
!! -499 to -200
!! -199 to 199
!! 200 to 499
!! ≥500
3. How many weeks was the child born? !! 37-38
!! 39-40
!! >41
4. In what season was the child born? !! Spring (March-May)
!! Summer (June-August)
!! Fall (September-November)
!! Winter (December-February) MOTHER
5. Mom's age at the time of birth? !! ≤24
!! 25-29
!! 30-34
!! ≥35 6. What kind of delivery did the mother have? !! normal way
!! caesarean 7. Did she have respiratory infections during her pregnancy? !! Yes
!! No
If yes, which? ………..
8. Did she take antibiotics during her pregnancy? !! Yes
!! No 9. Was she vaccinated during pregnancy? !! Yes
!! No
If yes, which? ………..
PEDIGREE
1. Atopic dermatitis 2. Allergic asthma 3. Allergic rhinitis
Lifestyle questionnaire Visit 1
Number of the clinical trial:
_________________
Date of birth of the parents:
Mother ____________ Father ______________
Profession of the parents:
Mother ____________ Father ________________
Postal code: ________________
Please check what is appropriate.
TO BE COMPLETED BY PARENTS
1. Where do you leave? In town
In the countryside
On a farm
2. Which level of education did the mother reach?
Basic education
High school
University of applied sciences
University
3. Which level of education did the father reach?
Basic education
Gymnase
High school
University
4. How many kids do you have? 1
2
3
4 or more
5. What is the yearly income of your household? (in CHF)
< 90 000
90 000-120 000
>120 000
6. Did you move less than a year ago in a Yes
No
freshly built/renovated home?
7. Did you renovate your home less than a year ago ?
Yes
No
8. Do you use the microwave more than 5 times per week?
Yes
No
9. Which material is covering the floor of your home? (except in the kitchen and the bathroom)
Only wood
Only floor tile
Only carpet
Only carpeting
Carpet covering the entire floor where your kid is sleeping
Other: …………..
10. In which frequency are you cleaning your home?
1x/week
1x/ 2 weeks
1x/ month
11. Is the level of humidity high in your home? (mold)
Yes
No
12. Do you have cockroaches at home? Yes
No
13. Do you have farm animals with furs or feathers?
Yes
No
If yes, which ones? ...
14. Do you have domestics animals with furs or feathers?
Yes
No
If yes, which ones? ...
15. Is there a smoker in your home? Yes
No
If yes, the person smokes:
At an open window
With the windows closed
TO BE COMPLETED BY THE MOTHER
16. Did you smoke during your pregnancy? Yes
No
17. Did you drink alcohol during your pregnancy?
Yes
No
18. Did you take fish oil 4g / day throughout your pregnancy?
Yes
No
19. Is there some home renovation inside your house during your pregnancy?
Yes
No
Life style questionnaire Visit 6
Number of the clinical trial:
_________________
Please check what is appropriate.
TO BE COMPLETED BY PARENTS
1. Do you use the microwave more than 5 times per week?
Yes
No
2. How frequently do you clean your home?
1x/week
1x/ 2 weeks
1x/ month
3. Is the level of humidity high in your home? (mold)
Yes
No
4. Do you have cockroaches at home? Yes
No
5. Do you have farm animals with furs or feathers?
Yes
No
If yes, which ones? ...
6. Do you have pets with furs or feathers? Yes
No
If yes, which ones? ...
7. Is there a smoker in your home? Yes
No
If yes, the person smokes:
At an open window
With the windows closed
8. How long has your child been hospitalized after birth?
Less than 10 days
Between 10 and 20 days
More than 20 days
9. Did your child have invasive procedures (central venous catheter, peripheral venous catheter, parenteral nutrition, ventilation, intubation, nasogastric tube, surgery) during his first weeks of life?
Yes
No
If yes, which ones? ...
10. Did your child have a positive allergy test with cats?
Yes
No
11. Did your child have a positive pollen allergy test?
Yes
No
12. Did your child have a positive allergy test with mites?
Yes
No
13. Did your child have a allergic rhinitis? No
Yes, diagnosed by a doctor
Yes, undiagnosed by a doctor
14. Did your child have a allergic asthma? No
Yes, diagnosed by a doctor
Yes, undiagnosed by a doctor
15. Did your child have a cold that lasted No
Yes 1x
more than 3 days? Yes 2x
Yes 3x or more
16. Did your child have a diarrhea that lasted more than 3 days?
No
Yes 1x
Yes 2x
Yes 3x or more
17. Did your child have a middle ear infection?
No
Yes 1x
Yes 2x
Yes 3x or more
18. Did your child have a pneumonia? No
Yes 1x
Yes 2x
Yes 3x or more
19. Did your child have chickenpox? Yes
No
20. How many times did you bring your child to the paediatrician due to an infection?
0
1x
2x
3x or more
21. Have you ever given antibiotics to your child?
Yes
No
If yes:
Local
Per os
22. Have you ever given antifungals to your child?
Yes
No
If yes:
Local
Per os
23. How many times has your child received the Di Te Pol Per vaccine?
0
1x
2x
3x
4x
24. How many times has your child received the Hib vaccine?
0
1x
2x
3x
4x
Life style questionnaire
Visit 9 and 11
Number of the clinical trial:
_________________
Please check what is appropriate.
TO BE COMPLETED BY PARENTS
1. Do you use the microwave more than 5 times per week?
Yes
No
2. How frequently are you cleaning your home?
1x/week
1x/ 2 weeks
1x/ month
3. Is the level of humidity high in your home?
(presence of mold)
Yes
No
4. Do you have cockroaches at home? Yes
No
5. Do you have farm animals with furs or feathers?
Yes
No
If yes, which ones? ...
6. Do you have pets with furs or feathers? Yes
No
If yes, which ones? ...
7. Is there a smoker in your home? Yes
No
If yes, the person smokes:
At an open window
With the windows closed
8. Did your child have a positive allergy test with cats?
Yes
No
9. Did your child have a positive pollen allergy test?
Yes
No
10. Did your child have a positive allergy test with mites?
Yes
No
11. Did your child have allergic rhinitis? No
Yes, diagnosed by a doctor
Yes, undiagnosed by a doctor
12. Did your child have allergic asthma? No
Yes, diagnosed by a doctor
Yes, undiagnosed by a doctor
13. Did your child have a cold that lasted more than 3 days?
No
Yes 1x
Yes 2x
Yes 3x or more
14. Did your child have a diarrhea that lasted more than 3 days?
No
Yes 1x
Yes 2x
Yes 3x or more
15. Did your child have middle ear infection? No
Yes 1x
Yes 2x
Yes 3x or more
16. Did your child have a pneumonia? No
Yes 1x
Yes 2x
Yes 3x or more
17. Did your child have chickenpox? Yes
No
18. How many times did you bring your child to the paediatrician due to an infection?
0
1x
2x
3x or more
19. Have you ever given antibiotics to your child?
Yes
No
If yes:
Local
Per os
20. Have you ever given antifungals to your child?
Yes
No
If yes:
Local
Per os
21. How many times has your child received the Di Te Pol Per vaccine?
0
1x
2x
3x
4x
22. How many times has your child received the Hib vaccine?
0
1x
2x
3x
4x
23. How many times has your child received the ROR vaccine?
0
1x
2x
Questionnaire on child nutrition Number of the clinical trial:
1. Do you breastfeed or did No
Yes, exclusively
you breastfeed your child?
If not or not exclusively, what extra milk did you give him?
Yes, but not exclusively
Hydrolyzed milk (ex: Alfaré, Damira, Pregomin)
Cow's milk
Milk with soy
Hypoallergenic milk ("HA" type)
Other: ...
2. How long did you breastfeed your child?
< 3 months
≥ 3 months
≥ 4 months
≥ 6 months
3. At what age did you introduce solid food to your child?
< 4 months (as currently recommended)
4-6 months
6-8 months
9 months
10 months or more
4. When did the child eat the following foods for the first time ?
- Egg - Fish - Cow milk - Kiwi - Sea food - Corn
- Nuts and hazelnuts - Peanuts
- Celery
<6months
<6months
<6months
<6months
<6months
<6months
<6months
<6months
<6months
6-12
6-12
6-12
6-12
6-12
6-12
6-12
6-12
6-12
>12months
>12months
>12months
>12months
>12months
>12months
>12months
>12months
>12months
5. Did your child have positive allergic tests for any of the following foods?
- Egg - Cow milk - Peanuts - Hazelnut - Corn
Yes
Yes
Yes
Yes
Yes
No
No
No
No
No