• Aucun résultat trouvé

Molecular characterisation of integrated sequences of Banana streak virus in the banana plant genome

N/A
N/A
Protected

Academic year: 2021

Partager "Molecular characterisation of integrated sequences of Banana streak virus in the banana plant genome"

Copied!
1
0
0

Texte intégral

(1)

Molecular characterisation of integrated sequences of Banana streak virus in the banana plant genome

Abstract of Poster Presentation

Molecular characterisation of integrated sequences of Banana streak virus in the banana plant genome

M. CHABANNES (1), P. Gayral (1), O. Guidolin (1), F. Baurens (2), S. Sidibe Bocs (2), N. Laboureau (1), M. Iskra-Caruana (1)

(1) CIRAD, UMR BGPI, F-34398 Montpellier Cedex 5, FRANCE; (2) CIRAD, UMR DAP, F-34398 Montpellier Cedex 5, FRANCE

Presenter’s E-mail: matthieu.chabannes@cirad.fr

The genome of banana (Musa sp.) harbours multiple integrations of several species of Banana streak virus (BSV),

certainly resulting from illegitimate recombination between host and viral DNA. Surprisingly, this pararetrovirus does not require integration for its replication. Some integrations, only existing in the Musa balbisiana genome (denoted B), are infectious by reconstituting a functional viral genome. To date, four widespread species of BSV (Goldfinger -BSGfV, Imové - BSImV, Mysore - BSMysV and Obino l’Ewai - BSOLV) have been reported as integrated into the B genome and as infectious, under stress conditions, resulting in viral infection of the banana plant. In order to study BSV expression from such viral integrants, a characterisation of infectious integrants (eBSV) was undergone by studying both a Musa BAC library obtained from the wild diploid M. balbisiana cv. Pisang Klutuk Wulung (PKW) containing the four BSV species described above and one interspecific genetic cross using carrier PKW. The organization of eBSGfV was fully characterized recently in our lab (Gayral et al., 2008). eBSGfV results from a single event of integration corresponding to an allelic insertion extensively rearranged, containing at least one full-length viral genome. Although the four BSV species present important differences with each other, the organisation of the three other eBSVs looks like eBSGFV. Indeed, preliminary data indicate that each of them is extensively rearranged in PKW and present as two insertions at the same locus. This suggests an allelic insertion resulting from a single even of integration. Experimental evidences to demonstrate BSV expression and to validate the infectious nature of every eBSV are on the way.

Références

Documents relatifs

Crops of Musa are susceptible to an ever increasing range of diseases requiring massive use of pesticides that have dramatic environmental and health impacts and threaten

Infectious endogenous sequences of several Banana streak virus species are present in the genome of Musa balbisiana spp, which are important progenitors for breeding improved

Infectious endogenous sequences of at least 3 distinct Banana streak virus species are present in the genome of Musa balbisiana spp [1, 2], which are important

The early evolutionary stages of infectious eBSV for BSGFV and BSImV were investigated among selected banana genotypes representative of the diversity of 60 wild Musa species and

No acti- vation of infectious eBSGFV (endogenous sequences of Banana streak Goldfinger virus) by tissue culture was observed in the two natural AAB plantain cultivars studied

eBSGfV results from a single event of integration corresponding to an allelic insertion extensively rearranged, containing at least one full-length viral genome. Although the four

Analysis of the segregation of these markers using a test-cross configuration allowed the construction of a genetic map of the linkage for the female parent (BB), containing the

Even though integration is not an essential step in the replication cycle of BSV, the nuclear genome of banana and plantain (genus Musa) contains viral integrations called