Distinct intracellular trafficking of hepatitis C virus in myeloid and plasmacytoid dendritic cells.

HAL Id: inserm-00517147

https://www.hal.inserm.fr/inserm-00517147

Submitted on 13 Sep 2010

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Distinct intracellular trafficking of hepatitis C virus in

myeloid and plasmacytoid dendritic cells.

Mélanie Lambotin, Thomas Baumert, Heidi Barth

To cite this version:

Mélanie Lambotin, Thomas Baumert, Heidi Barth. Distinct intracellular trafficking of hepatitis C virus in myeloid and plasmacytoid dendritic cells.. Journal of Virology, American Society for Microbiology, 2010, 84 (17), pp.8964-9. �10.1128/JVI.00517-10�. �inserm-00517147�

A

Fig. 1, Lambotin et al.

CD11c

IL-3R

BDCA2

pDCs

CD80

CD86

mDCs

B

pDCs

mDCs

SR-BI

CLDN1

CD81

DC-SIGN

C

0 1 2 3 4 5 6 7 8 9 10 11 12

CD81

SR-BI

CLDN1

MFI-Rate

pDCs

mDCs

p=0.023 n.s. n.s.

Fig. 2, Lambotin et al.

number

of

E2

positi

v

e

cells/

total

number

of

cells

(n=300)

100 120 0 20 40 60 80

pDCs mDCs

p = 0.012

D

E

%

inhibitio

n

of

HCVcc

utpake

pDCs

mDCs

%

inhi

bitio

n

of

HCV

cc

utpa

ke

0 20 40 60 80 100 0 20 40 60 80 100

A

_pDCs

_mDCs

Copies

HCV RNA

/

µg cel

lular

RNA

10

7

10

6

10

5

10

4

10

3

10

2

10

1

10

0

10

7

10

6

10

5

10

4

10

3

10

2

10

1

10

0

Copies

HCV RNA

/

µg cel

lular

RNA

pDCs

mDCs

+ HCVcc

B

NC

C

mDCs

Anti-E2

Anti-core

overlay

Fig. 3, Lambotin et al.

pDCs

mDCs

A

HCV E2 Rab5 Overlay HCV E2 Lamp1 Overlay HCV E2 CD71 Overlay HCV E2 Rab5 Overlay HCV E2 Lamp1 Overlay HCV E2 CD71 Overlay

B

CD71 MHC I Overlay CD71 MHC II Overlay Lamp1 MHC I Overlay Lamp1 MHC II Overlay