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Titania–hydroxyapatite nanocomposite coatings support human mesenchymal stem cells osteogenic differentiation

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FIGURE 1. Chemical characterization of TiO 2 interaction with HA via energy-dispersive X-ray (EDX) line scan analysis of TiO 2 –HA coatings cross- cross-section at the coating elemental interfacial region upper layers is shown
FIGURE 3. Three-dimensional visualizations of the nano-roughness of TiO 2 –HA nanocomposite (left) compared with the pure TiO 2 (right)
Figure 5 shows the levels of two important markers of osteoblast activity, 23,24 ALP and OC, after culture of hMSC and hMSC-ob on the different coatings
FIGURE 5. Osteoblastic markers in hMSC and hMSC-ob cultured on nanocomposites. Cells were incubated up to 21 days on the different surfaces and alkaline phosphatase (ALP) activity (a) and osteocalcin (OC) expression (b) were measured in cell lysates
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