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Find human protein sequence FGF3

• What is the accession number and function of this protein?

• How many valines does the sequence contain?

• How long is the longest fragment after trypsin digestion?

• How many human similar reference proteins are in the databases?

• Does the sequence contain any transmembrane regions?

Download seqence NM_001757.3

• What does this sequence encode ?

• Is there a complementary sequence to primer R1?

>R1

GCTCTGACGCTCATGATGC

• In which exon is the complemetary sequence for this primer?

• Is the primer suitable for PCR with Ta=60°C?

• Design suitable F primer for this experiment.

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Version 2

download sequence NM_005247.2

• What does this sequence encode?

• Are there any significant single nucleotide polymorphisms (GMAF), in which exons?

• Would be the restriction endonucleases NdeI and KpnI suitable for cloning of the CDS?

• Translate the CDS of this sequence into the protein sequence, write down the protein sequence in FASTA format.

• Compare the protein to its homologue from mouse (Mus musculus), what is their identity?

Download the peptide sequence S2.

>S2

CGPSTSGTTSGPGPARPARARPRRPREETLTPEEEEKRRVRRERNKLAAAKCRNRRRELT DRLQAETDQLEEEKAELESEIAELQKEKERLEFVLVAHKPGCKIPYEEGPGPGPLAEVRD LPGSAPAKEDGFSWLLPPPPPPPLPFQTSQDAPPNLTASLFTHSEVQVLGDPFPVVNPSC

• Identify the protein.

• Does it contain any typical domains?

• How many cysteins contain the peptide?

• What is the molecular weight of this peptide?

• Is the sequence (whithin the compared area) identical to the identified protein?

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