HAL Id: hal-02616080
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Quantification of peptide adsorption on lipid
nanocapsules without prior purification or separation process
Claire Gazaille, Marthe Akiki, Joël Eyer, Guillaume Bastiat
To cite this version:
Claire Gazaille, Marthe Akiki, Joël Eyer, Guillaume Bastiat. Quantification of peptide adsorption on lipid nanocapsules without prior purification or separation process. Journée scientifique de la SFR ICAT 4208, Jun 2019, Angers, France. 2019. �hal-02616080�
0 0,3 0,6 0,9 1,2 1,5 1,8 2,5 3,5 4,5 5,5 6,5 7,5 8,5 In tensity ( without unity) Times (minutes)
Quantification of peptide adsorption on lipid nanocapsules
without prior purification or separation process
Gazaille C, Akiki M, Eyer J, Bastiat G*
Translational Micro and Nanomedicines (MINT, INSERM 1066 / CNRS 6021), UNIV ANGERS, Angers, France
*corresponding author: guillaume.bastiat@univ-angers.fr
Incubation overnight under magnetic stirring
Figure 1. Chromatogram showing the retention times of LNCs
(3.5 minutes) and NFL (7.7 minutes) on size exclusion column.
Better NFL adsorption with
negatively charged LNCs.
Results consistent with the positive charge of NFL.7
Hypothesis : electrostatic
interactions between NFL
and LNCs.
1. LNC formulation
5,6To quantify the encapsulation rates of active agents (drug, protein, etc.) in nanoparticles, the separation of non-loaded agents from the loaded ones
is often reported in literature, using dialysis or centrifugal filters.1,2 However, these techniques are more and more questioned, and the low quality of
the separation could lead to over or underestimated data.3,4 To avoid the biases, a method was developed to quantify free peptide proportions
without prior separation step from peptide adsorbed at the nanoparticle surface, using NFL-TBS.40-63 (NFL) and lipid nanocapsules (LNCs) as
peptide and nanoparticle models, respectively.
75°C 45°C Addition of deionized water Oil/Water emulsion
Phase inversion zone
Components : • Oil : Labrafac® • Surfactants : Span® 80, Kolliphor® • De-ionised water • NaCl Water/Oil emulsion LNCs in suspension
2. NFL adsorption on LNCs
Better NFL adsorption with
high Span® 80 composition
at LNC surface.
When Span® 80 composition increases, NFL adsorption is enhanced.
Hypothesis : hydrogen
bonds between the NFL and
Span® 80 at LNC surface.
Figure 3. Influence of Span® 80 composition
of LNCs on NFL adsorption. LNCs were used at 0.975 mg/mL and NFL at 0.1 mg/mL. The LNC size corresponds to 50 nm. (Mann-Whitney test ; ** p < 0.01 ; ns p > 0.05 ; n = 4-5 ; mean ± SD).
Figure 4. Influence of LNC size on NFL
adsorption. LNCs were used at 0.975 mg/mL and NFL at 0.1 mg/mL. (Mann-Whitney test ; * p < 0.05 ; ns p > 0.05 ; n = 4-5 ; mean ± SD)
3. Influence of LNC charge on NFL adsorption
4. Influence of LNC composition on NFL adsorption
5. Influence of LNC size on NFL adsorption
When the LNC surface increases, NFL adsorption is enhanced.
Thanks to a size exclusion method, the separation between nanoparticles and active agent and their quantification are performed simultaneously.
This single step is a real benefit for analysis accuracy and robustness, minimizing the biases compared to sequential steps for separation and
quantification. In this particular case, the best combinations of concentrations/types of LNCs and NFL were determined to achieve a total NFL
adsorption at LNC surface. This technique could be a promising tool to characterize other nanoparticle/active agent interactions.
6. Interaction inhibition between NFL and LNCs
Inhibition of NFL adsorption
at LNC surface when NaCl and urea are present.
Proof of electrostatic
interactions and hydrogen
bonds between LNCs and
NFL.
Figure 5. Inhibition of interactions between NFL and LNCs in presence of NaCl and urea. LNCs were used at 9.75 mg/mL and NFL at 0.1 mg/mL. The LNC size corresponds to 50 nm. (n = 3 ; mean ± SD)
References
1. Chen, L. et al. Int. J. Biol. Macromol. 2019, 254–261. 2. Abriata, J. P. et al. Mater. Sci. Eng. C.2019, 347–355.
3. Johnsen, E. et al. J. Pharm. Biomed. Anal. 2016, 106–111. 4. Garvin, A. et al. J. Forensic Sci. 2013, S173–S175.
5. Heurtault, B. et al. Pharm Res. 2002, 875-80.
6. Heurtault, B. et al. Biomaterials. 2003, 4283–4300. 7. Berges, R. et al. PLoS One. 2012, e49436.
Acknowledgments 1.8 1.5 1.2 0.9 0.6 0.3 2.52.5 3.53.5 4.54.5 5.55.5 6.5 7.5 8.5
Figure 2. Influence of LNC charge on NFL
adsorption. LNCs were used at 0.975 mg/mL and NFL at 0.1 mg/mL. Negative and positive LNCs are respectively with 2.5 % of S-Chol and 5 % of DDAB (w/wLab). The LNC size corresponds to 50 nm. (Mann-Whitney test ; * p < 0.05 ; ** p < 0.01 ; n = 4-5 ; mean ± SD)
LNCs with high Span® 80 proportion (15 % (w/wtotal))
Negative LNCs (2.5 % S-Chol (w/wLab)) without Span® 80
