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Improving immune competence: screening for new parameters describing the innate immune status in chicken

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HAL Id: hal-01193926

https://hal.archives-ouvertes.fr/hal-01193926

Submitted on 6 Jun 2020

HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci- entific research documents, whether they are pub- lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

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Improving immune competence: screening for new parameters describing the innate immune status in

chicken

Fanny Calenge, Pascale Quéré, Sascha Trapp, Sandrine Grasteau, Elisabeth Duval, Irène Gabriel, Bertrand Bed’Hom

To cite this version:

Fanny Calenge, Pascale Quéré, Sascha Trapp, Sandrine Grasteau, Elisabeth Duval, et al.. Improving immune competence: screening for new parameters describing the innate immune status in chicken.

8. European Symposium on Poultry Genetics (ESPG), Sep 2013, Venise, Italy. �hal-01193926�

(2)

IMPROVING IMMUNE COMPETENCE: SCREENING FOR NEW PARAMETERS DESCRIBING THE INNATE IMMUNE STATUS IN CHICKEN

F. CALENGE1,2*, P. QUÉRÉ3,4, S. TRAPP3,4, S. GRASTEAU5, E. LE BIHAN-DUVAL5, I. GABRIEL5, AND B. BED’HOM1,2

INTRODUCTION

Introducing new parameters related to immune competence in selection programs could counterbalance some of the deleterious consequences on health and welfare of the intense selection conducted so far for chicken production- related traits. An ongoing project, described below, is aiming at both developing non invasive and rapid methods to describe innate immunity (i.e. by using whole blood samples) and at describing the correlations of innate immunity with digestive efficiency and gut microbiota composition.

PROJECT DESCRIPTION

HOST GENETICS

Group D+

HIGH DIGESTIBILITY

Group D-

LOW DIGESTIBILITY

MEASURING GUT MICROBIOTA COMPOSITION Will differences in microbiota composition previously demonstrated between lines D+

and D- be confirmed between these groups with16S rDNApyrosequencing?

GUT MICROBIOTA COMPOSITION

Microbiota composition

n°1

Microbiota composition

n°2

≠ ?

≠

MEASURING INNATE IMMUNITY

INNATE IMMUNITY

Immune

parameters 1

≠ ?

parameters 2Immune

Commercial broiler line ANIMAL MATERIAL

Two lines with divergent digestive efficiencies selected from a commercial broiler line were crossed to produce AIL. Two-hundred AIL will be screened for their digestive efficiency and divided into two groups of 30 animals with contrasted digestibilities (groups D+/D-) .

Line D+ Line D-

AIL

Advanced Intercross Lines

DIGESTIVE EFFICIENCY

? ?

?

CALCULATING CORRELATIONS

1 INRA. UMR1313 Génétique Animale et Biologie Intégrative. Domaine de Vilvert. 78352 Jouy-en-Josas. France

2 INRA. UMR1313 AgroParisTech. Domaine de Vilvert. 78352 Jouy-en-Josas. France

3 INRA. UMR1282 Infectiologie et Santé Publique. 37380 Nouzilly. France

4 INRA. UMR1282 Université François Rabelais. 37000 Tours. France

5 INRA. UR83 Unité de Recherches Avicoles. 37380 Nouzilly. France

MEASURING INNATE IMMUNITY Are parameters describing innate immunity differing between both groups? Quantification of innate immune gene expression in whole blood and of blood leucocyte and thrombocyte populations.

CONCLUSION

This descriptive project will allow a better understanding of the complex interactions of innate immmunity with gut microbiota and digestibility. Following projects will assess which of the parameters describing innate immunity or gut microbiota composition are predictive of the animal’s immune competence after challenges with pathogens. On the other hand, the role of host genetics will be investigated to identify host genes predicting the immune competence, which could be selected to produce chicken with improved immune competence.

CALCULATING CORRELATIONS How are parameters describing innate immunity, digestive efficiency and gut microbiota composition correlated between bothgoups?

Do these correlations reveal potential interactions between these traits?

Références

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