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Characterization of N-glycans from mouse brain neural cell adhesion molecule

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Academic year: 2021

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Fig. 1. Characterization of NCAM from mouse brain by SDS–PAGE and Western blotting. (A) silver stained SDS-gel; (B–D) Western blot analyses employing anti-NCAM mAb H28 (B), anti-PSA mAb 735 (C), and anti-HNK1 mAb 412 (D)
Fig. 2. Strategy for analysis of glycans and peptide moieties from mouse NCAM.
Table I. Characterization and assignment of tryptic, deglycosylated PSA peptides after separation by RP-HPLC
Fig. 5. MALDI-TOF mass spectra of native desialylated PSA glycans. Glycans were analysed in the positive-ion (A) or negative-ion (B) reflectron mode and were recorded as [M+Na] + or [M-H] – pseudomolecular ions, respectively.
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