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Mitochondrial sphingosine-1-phosphate lyase is essential for phosphatidylethanolamine synthesis and survival of Trypanosoma brucei

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Academic year: 2021

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Figure S1:  Verifying the TbSpl KO cell line.  a)  Genomic DNA of parental 29-13  wild-type strain (+/+), single- (+/-) and double-allele (-/-) knockout cells was extracted
Figure S2: Metabolic labeling with  3 H-serine. Cells were cultured for 4, 6 or 8 days  in the presence (+ tet) or absence (- tet) of tetracycline and labeled with  3 H-serine for  the last 24 hours
Figure S3:  Assessment of mitochondrial membrane potential. a)  TbSpl KO  parasites were depleted of TbSpl for 0  (control), 6 or 8 days and stained with the  mitochondrial membrane potential-dependent dye MitoTracker Red

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