Comparative trials for the detection of CTV antisera from different sources Boubker J., Kyriakou A.P., D' Onghia A.M., Baloglu S., Yilmaz M.A. in

Boubker J., Kyriakou A.P., D' Onghia A.M., Baloglu S., Yilmaz M.A.

in

Martelli G.P. (ed.), D' Onghia A.M. (ed.).

Proceedings of the Mediterranean network on certification of citrus. 1995-1997 Bari : CIHEAM

Options Méditerranéennes : Série B. Etudes et Recherches; n. 21 1998

pages 113-118

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--- Boubker J., Kyriakou A.P., D 'Onghia A.M., Baloglu S., Yilmaz M.A. Comparative trials for th e detection of CTV an tisera from differen t sou rces. In : Martelli G.P. (ed.), D 'Onghia A.M. (ed.).

Proceedings of the Mediterranean network on certification of citrus. 1995-1997. Bari : CIHEAM, 1998. p.

113-118 (Options Méditerranéennes : Série B. Etudes et Recherches; n. 21)

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J . Boubker

-

A.

Agricultural

- Cyprus

Agronomic Bari

S. Baloglu, A. Yilmaz

Çukurova University, Faculty Agriculture, Department

Adana

Turkey

-

monoclonal and two polyclonal, in enzyme-linked assay

tests against CTV isolates counties:

and the exception of one monoclonal which failed to

a few isolates and the effective in CTV diagnosis

which implies that the testing in

words: diagnosis, counties

-

agrumes, tristeza, closterovirus, diagnostic,

Options Méditerranéennes, Série B /n021, - Proceedings the Mediterranean Network on Certification Citrus

114 J . Boubker, A. A. D’Onghia, S. Baloglu, A. Yilrnaz

a in by aphids, is

one of the most of which has caused the death of millions

of on (Citrus aurantium) in Latin and

including

The disease has also affected in the Spain et al., 19SS),

et al., 1976), et al., 1993).

et al. ).

The of CTV in the in combination with the use of the most

sensitive the disease, and the existence of Aphis gossypii, a of the a

to in is a in to

of symptoms induced. symptomless to causing decline and

death of the plants. The seedling yellows which is the found

in Spain et al., 1988).

techniques have been employed the diagnosis of CTV, including biological indexing

the use of plants, lime (C. aurantifolia), the method

chain

1991). biological the methods which have been most

commonly applied’ on a basis CTV detection in Joseph et al., 1979).

to CTV, polyclonal monoclonal, being used in

diagnosis of the the of each of the above

in detecting not well known. Within the context of the

activities of the on of of

polyclonal effectiveness in the

diagnosis of local CTV isolates in

~

aterials and methods

i .

l

Samples of in Spain

almost in the of Adana

isolates of CTV using

(Table The which came the companies of and

polyclonal, the Spanish The in all

each set of was that suggested by the

Comparative trials for the detection of by antisera from different sources 115

Table - CTV infected isolates tested in

of Cukurova Üniversity,

-

-

-

-

- -

4gn"ultural Agronomico

de la des Végétaux, des Controles Téchniques et de la des

Total samples

16

12 5

12

Code

T13, T12, T21 1, 42A,

GY 1- 12

T4, LG1, LG2,

cuttings of to be CTV-

infected, collected in the field the of negative

used in each

1 : l O filled with plant

sap of each isolate.

the at the addition of the

positive value was at least twice as high as that of the

All effective in the detection of most CTV isolates tested. The

two out of 16 5) and one out 12 isolates

the of the isolates

the Spain. The of the

is the

the in Adana and Nicosia the polyclonal

gave the 405 nm.

in the diagnosis of all the high specificity and the consistency

116 J. Boubker, A. A. D'Onghia, S. Baloglu, A. Yilmaz

in quality of be A use of a

monoclonal and a of in

40 35

20 15 10 5 O

1 2 3 4 5 6 7 8 9 1 0 1 1 1 2

CTV isolates

Spain

1

30 25 20 15 10 5 O

1 2 3 4 5 6 7 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 isolates

,"ab-l ,

I

of against CTV isolates (A) and

(B) in tests.

Comparative for the detection of CTV by fiom dyerent sources 117

IMMabs-l Monocl. antib. from Spain DMabs-2 Monocl. antib. from Morocco Pabs-l Polyclonal antib. from France Pabs-2 Polyclonal antib. from Switzerland l

25

~~

20

h

45 15

* B

4 g 10 5

O

- of CTV detection in

J.F., J.A. and L. (1988). of a - seedling yellows

Spain. Con$ Spain, pp. 28-32.

Nitzan, Y. (1991). and of isolates in

seedlings. Con$ pp. 162-165.

Loebenstein, G. (1979). The use of detection of

69: 190-194.

(1995). of in the East. Virus and vinrs-like

diseases citrus in the region, eds, pp. 55-92.

F., A in

of Cor$

12"' Con$

J. of

In Con$ Spiin, pp. 71-76.

to of on FAO

1641,16 pp.

118 J . Boubker, A . A . D'Onghia, S. Baloglu, A . Yilmaz

F. and F. (1958). of disease in Egypt. FAO Bull.

Vol. 6 (lo), pp. 153-54.

Loebenstein, and Y. (1976). of by aphids

on and of the in 7'' Cor$

pp. 47-49.

C.N. (1991). Graf-transmissible diseases citrus. Handbook detection and diagnosis. FAO eds, pp. 17-33