• Aucun résultat trouvé

Inducible Treg cell populations as cell based-therapy for rheumatoid arthritis

N/A
N/A
Protected

Academic year: 2021

Partager "Inducible Treg cell populations as cell based-therapy for rheumatoid arthritis"

Copied!
2
0
0

Texte intégral

(1)

HAL Id: inserm-00758221

https://www.hal.inserm.fr/inserm-00758221

Submitted on 28 Nov 2012

HAL is a multi-disciplinary open access

archive for the deposit and dissemination of

sci-entific research documents, whether they are

pub-lished or not. The documents may come from

teaching and research institutions in France or

abroad, or from public or private research centers.

L’archive ouverte pluridisciplinaire HAL, est

destinée au dépôt et à la diffusion de documents

scientifiques de niveau recherche, publiés ou non,

émanant des établissements d’enseignement et de

recherche français ou étrangers, des laboratoires

publics ou privés.

Inducible Treg cell populations as cell based-therapy for

rheumatoid arthritis

Delphine Martire, Julie Quentin, Anne-Laure Mausset-Bonnefont, Hélène

Asnagli, Nathalie Belmonte, Arnaud Foussat, Christian Jorgensen, Pascale

Louis-Plence

To cite this version:

Delphine Martire, Julie Quentin, Anne-Laure Mausset-Bonnefont, Hélène Asnagli, Nathalie Belmonte,

et al.. Inducible Treg cell populations as cell based-therapy for rheumatoid arthritis. 7th European

Workshop on Immune-Mediated Inflammatory Diseases, Nov 2012, Noordwijk aan Zee, Netherlands.

pp.P55. �inserm-00758221�

(2)

P O S T E R P R E S E N T A T I O N

Open Access

Inducible Treg cell populations as cell

based-therapy for rheumatoid arthritis

Delphine Martire

1

, Julie Quentin

1

, Anne-Laure Mausset-Bonnefont

1

, Hélène Asnagli

2

, Nathalie Belmonte

2

,

Arnaud Foussat

2

, Christian Jorgensen

1

, Pascale Louis-Plence

1*

From 7th European Workshop on Immune-Mediated Inflammatory Diseases

Noordwijk aan Zee, the Netherlands. 28-30 November 2012

Background

Adoptive cell transfer of Treg cells is a promising approach to restore tolerance in autoimmune disease.

However the various type of Tregs, their doses of injection and their in vivo-suppressive mechanism need to be precisely define to clearly establish which Tregs will be able to dampen efficiently the immune response in the various settings.

In our study, we compared the therapeutic potential of induced CD25+FoxP3+ and two IL10-secreting Tregs: Tr1 and CD49b-induced Tregs.

Materials and methods

CD49b Treg cells were generated in naive mice following repetitive injections of iDC. The purification was based on the negative selection of CD4 T cells isolated from the spleen and liver of the iDC-vaccinated mice.

Cell sorting experiments were realized to obtain 98% pure CD49b+T or CD25+cells. Collagen type II (bCII) specific Tr1 clones were obtained from TCR transgenic mice and expandedin vitro. Selected clones showed in vitro antigen specificity, Tr1 cytokine profile and IL10-and TGFb-dependent suppressive activity.

Results

Several doses of CD49b or Tr1 cells were injected i.v. at day 28 in established collagen-induced arthritis. Clinical signs of arthritis were scored, as well as biological para-meters such as the level of anti-bCII antibodies in sera and the cytokine profile of bCII specific T cells.

We defined for both Treg cell populations the dose effect in curative settings experiments. One single dose of 3x106 or 1x106 of Tr1 cell administration could reduce

the incidence and severity of CIA. Interestingly, higher dose of 10M of Tr1 cells did not improve the disease. In the same manner, the dose of 105CD4CD49b+or CD25+ cells reverse clinical symptom with a lack of efficacy of higher doses. The cytokinic profile of the Tr1 cells was also investigated in inflammatory settings as well as the impact of the various Treg cells on the proliferation of effector cellsin vivo.

Conclusions

Our results suggest that even if the Treg cells present some similarities, we need to precisely define the dose and type of Treg that will be efficient in each experi-mental setting.

Author details

1Inserm U844, Université Montpellier 1, CHU Lapeyronnie, Montpellier, France.2TxCell, Valbonne-Sophia Antipolis, France.

Published: 28 November 2012

doi:10.1186/1479-5876-10-S3-P55

Cite this article as: Martire et al.: Inducible Treg cell populations as cell based-therapy for rheumatoid arthritis. Journal of Translational Medicine 2012 10(Suppl 3):P55.

1Inserm U844, Université Montpellier 1, CHU Lapeyronnie, Montpellier, France Full list of author information is available at the end of the article

Martire et al. Journal of Translational Medicine 2012, 10(Suppl 3):P55 http://www.translational-medicine.com/content/10/S3/P55

© 2012 Martire et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Références

Documents relatifs

[r]

loin d’avoir cette attitude fraternelle, intrigue pour susciter des ennemis à l’empire, pour l’encercler, et va si loin dans cette voie que le chan­ celier se

To determine the suitability of a software development method- ology (SDM) to aid in the development of location-based games, it is nec- essary to determine to what degree SDMs

(This could represent a majority of those personnel who had to use the phone.) Another difficulty with the emergency phone arose when floor wardens used it unnecessarily and thus

Control for Dynamic Positioning and Way- point Tracking of Underactuated Autonomous Underwater Vehicles Using Sliding Mode Control.. The MIT Faculty has made this article

Residents of larger, wealthier municipalities with more formalized labour experienced more pronounced reductions in daily distance travelled during the lockdown period.. (a)

In addition to a new operating fund formula, DHCD must lobby for increased funding levels from the state legislature to successfully implement an asset management model

In microsatellite analysis, moderate genetic diversity values were found for Madagascar, together with low mean number of alleles ranging from 2.47 to 3.88 compared to South