HAL Id: hal-02817713
https://hal.inrae.fr/hal-02817713
Submitted on 6 Jun 2020
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Molecular bases of acclimation to water deficit in poplar mature leaves
David Cohen, Irène Hummel, Sandrine Balzergue, Marie-Laure Martin-Magniette, Emilie Tisserant, Didier Le Thiec, Erwin Dreyer,
Jean-Pierre Renou, Marie-Béatrice Bogeat-Triboulot
To cite this version:
David Cohen, Irène Hummel, Sandrine Balzergue, Marie-Laure Martin-Magniette, Emilie Tisserant,
et al.. Molecular bases of acclimation to water deficit in poplar mature leaves. Plant Abiotic Stress
Tolerance. International conference, Feb 2009, Vienne, Austria. 1 p., 2009. �hal-02817713�
Water deficit markers
The co-analysis of two poplar clones of contrasted tolerance allowed us to discriminate :
9 differentially expressed genes showing a potential adaptative character from genes responding passively to water deficit 9 good water deficit markers which are regulated both in Carpaccio and Soligo.
Affymetrix array results are currently under validation by real-time PCR and by array on an independent experiment.
Candidate genes for water deficit acclimation (Carpaccio specific response) will be validated through an analysis of expression in a wider range of clones by real-time PCR.
Affymetrix arrays are currently performed on other organs such as root apices and stomata to allow meta-analyses.
Molecular
Molecular bases of acclimation bases of acclimation to water
to water deficit deficit in poplar in poplar mature leaves mature leaves
D Cohen, I Hummel, D Le Thiec, E Dreyer, MB Bogeat-Triboulot, UMR1137 EEF, INRA Nancy, France JP Renou, S Balzergue, ML Martin-Magniette, URGV Evry , France
E. Tisserant, UMR1136 IAM , INRA Nancy , France
Projet POPSEC – ANR Génoplante GPLA06028G– Vienne 2009
Carpaccio exclusive response Unknown proteinEAR AMI AMO (2) ABA 8'-hydroxylase (2)
ABC transporter family protein Receptor-like protein kinase
LRR receptor-like protein kinase 1 / NBS-LRR type
0 10 20 30 40 50 60 70 80
<1 [1,1.5[ [1.5, 2[ [2, 2.5[ [2.5,3[ >3 0
10 20 30 40 50 60 70 80 0 10 20 30 40 50 60 70 80
Regulated genes: number and intensity
Material and Methods:
Experience was conducted on two genotypes of Populus deltoides x nigra, Carpaccio and Soligo, the later being more sensitive to water deficit (Moncluset al. 2006). The transcriptome of mature leaves was analysed at different stages and intensities of water deficit (early or following 12 days acclimation; mild and moderate, figure below).
Transcriptome was analysed on the GeneChip Poplar Genome Array (URGV Evry, France) using 2 replicates (a pool of 3 trees each) per modality. Re-annotation of Affymetrix probesets was performed based on poplar gene-models annotation (JGI).
Context:
In temperate zones, global change will induce longer and more severe drought episodes. In order to identify candidate genes for water deficit acclimation, we looked for gene expression in response to drought stress in poplar, a model tree species.
Objectives:
- Identify strong drought markers by comparing the response of two genotypes at different stages and intensities of water deficit
-Identify candidate genes for drought acclimation
Differentially expressed genes in the two clones for each water deficit
EAR AMI AMO EAR AMI AMO
-300 -200 -100 0 100 200
300 up
down
• Soligo response to AMI was the weakest.
Response regulator 5 (ARR5) NBS-LRR type
LEA 5 Glutamine synthetase Serine hydroxymethyltransferase UDP-glucosyl transferase family protein polyubiquitin senescence-associated protein Proton-dependent oligopeptide transport (2) AREB / ABA-induced-like protein
Aquaporin TIP / PIP ERD protein-related Galactinol synthase 2 NADPH oxidase LEA (4)
SNARE-interacting protein Rapid alkalinization factor putative serine protease (2)
351 106 219
33 183 340
9-cis-epoxycarotenoid dioxygenase (5) AREB2 bZip (2,1) WRKY (2) calmodulin-binding protein (2)
ERF RWP-RK domain-containing protein
Gibberellin regulated protein ChaC-like family protein-like (3)
RAV1/ WRKY (2)
Zinc finger homeodomain LTP
Photosystem family proteins (3) Kelch repeat-containing protein Ubiquitin family protein (2) 4-coumarate-CoA ligase-like protein AP2-EREBP
482 105 295
Kelch repeat-containing F-box family protein unknown protein
Polyphenol oxidase Alcohol dehydrogenase WAK-Like (6) Sucrose synthase (3) LRR family protein (6) LTP
Fructose-6-phosphate 2-kinase DEAD/DEAH box helicase (3) Isoamylase
laccase
kelch repeat-containing protein NBS-LRR class (6) / LRR-kinase (3) RD22-like protein
ChaC-like family protein-like Sucrose synthase Hexose transporter
Probesets specifically regulated in the early response
38 7 [11]
87 36 [27]
Acclimation to water deficit
Moderate water deficit 65
63 [36]
71 78 [28]
2 15 [2]
13 43 [7]
2 [1]
Both are down regulated in tolerant clone and up-regulated in the more sensitive one
Mild water deficit
[unkown protein]
EAR
AMI
AMO
Probesets distribution by classes of Log ratio Intensity (absolute value)
number
Percent
•For both clones, early responses mainly involved up-regulations, whereas long term water deficits repressed
gene expression.
• Carpaccio exhibited a less numerous but a stronger gene regulation than Soligo.
Up-regulation Down-
Only in the most tolerant clone Common to both clones
Genes commonly regulated by water deficit in A. thaliana (Bray , 2004)
Homeobox-leucine zipper protein Unknown protein (2)
Galactinol synthase 1 RCI 2A - like
Protein phosphatase 2C (2) Xyloglucan endotransglycosylase
*
*
*
*
*
Regulated in the 3 treatments
