HAL Id: hal-02955098
https://hal.inrae.fr/hal-02955098
Submitted on 1 Oct 2020
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Exploring the impact of ZmMYB31 overexpression in maize under contrasted soil water regimes
Laetitia Virlouvet, Charlotte Henriet, Marie-Pierre Jacquemot, Sandrine Chaignon, Thierry Balliau, Marie Javelle, Christophe Sallaud, Sandrine
Balzergue, Marie-Laure Martin-Magniette, Johann Joets, et al.
To cite this version:
Laetitia Virlouvet, Charlotte Henriet, Marie-Pierre Jacquemot, Sandrine Chaignon, Thierry Balliau, et al.. Exploring the impact of ZmMYB31 overexpression in maize under contrasted soil water regimes.
WG2 meeting of the COST FA1306. Driving integrative cell phenotyping through “omics”., Feb 2016, Versailles, France. �hal-02955098�
Exploring the impact of ZmMYB31 overexpression in maize under contrasted soil water regimes
Laëtitia Virlouveta, Charlotte Henrieta, Marie-Pierre Jacquemota, Sandrine Chaignona, Thierry Balliaub, Marie Javellec, Christophe Sallaudc, Sandrine Balzergued, Marie-Laure Martin- Magnietted,e, Johann Joetsb, François Tardieuf, Claude Welckerf, Michel Zivyb, Valérie Méchina, Sylvie Coursola
aInstitut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, F-78026 Versailles Cedex, France; bGénétique Quantitative et Evolution - Le Moulon, INRA, Univ Paris-Sud, CNRS, AgroPArisTech, Université Paris-Saclay, F-91190 Gif-Sur-Yvette, France ; cBiogemma, Centre de Recherche, F-63720 Chappes, France; dInstitute of Plant Sciences Paris-Saclay IPS2, CNRS, INRA, Univ Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, Rue de Noetzlin, F-91405 Orsay, France;
eUMR MIA-Paris, AgroParisTech, INRA, Université Paris-Saclay, F-75005 Paris, France ;
fINRA, UMR 0759, Laboratoire d’Ecophysiologie des Plantes sous Stress Environnementaux, F-34060 Monptellier Cedex 1, France
Water deficit directly impacts the ability of plants to intercept and convert light into biomass.
Because leaf growth is one of the first processes affected by water deficit, many physiological studies concentrated in short-term responses and associated mechanisms. They demonstrated the roles of cellular and metabolic processes such as changes in cell turgor, hydraulic conductance and cell wall plasticity. However, our understanding of how water deficit impacts the cell wall biosynthesis and dynamics is still fragmentary. Here, we report that ZmMYB31, a subgroup 4 R2R3-MYB transcription factor that acts as a repressor of the lignin pathway in Arabidopsis [1-2], is induced by water deficit in the leaf growing zone and colocates with QTLs for lignin content and growth responses to water deficit in maize. To assess the role of ZmMYB31 in maize upon water limitation, we generated maize transgenic lines overexpressing ZmMYB31. We showed, in preliminary experiments, that the leaf growing zone of T1 ZmMYB31 overexpression plants had a higher content of β-O-4-linked lignin units than that of wild-type sister plants grown in the greenhouse under well-watered conditions. A comparative analysis by quantitative RT-PCR revealed significant differences for six lignin biosynthetic genes in the leaf growing zone of T1 ZmMYB31 overexpression plants compared with that of wild-type plants, consistent with the observed changes in -O-4- linked lignin unit content. To obtain additional clues with regard to ZmMYB31 function in maize under water-limited conditions, we are currently combining RNA-seq analysis and shotgun proteomics of the leaf growing zone of homozygous ZmMYB31 overexpression plants and their wild-type siblings grown in the PhenoArch platform under well-watered and moderate water deficit conditions. Integrative statistical approaches shall allow us to infer a ZmMYB31-regulated network and identify variables underpinning maize responses to water deficit.
Keywords: maize, water deficit, ZmMYB31, lignin biosynthesis, omics
[1] Fornalé S, Sonbol FM, Maes T, Capellades M, Puigdomenech P, Rigau J, Caparros-Ruiz D (2006) Plant Mol. Biol. 62: 809-823.
[2] Fornalé S, Shi X, Chai C, Encina A, Irar S, Capellades M, Fuguet E, Torres JL, Rovira P, Puigdomenech P, Rigau J, Grotewold E, Gray J, Caparrós-Ruiz D (2010) Plant J. 64: 633-644.
