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export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-Like transporter EDS5

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Academic year: 2021

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Supplementary Fig. 1. Yeast background and EDS5 SA transport is disrupted by ionophores. Whole yeasts

were loaded with labeled SA (14C-SA) in the presence and absence of the ionophores, nigericin and CCCP, and net SA retention was quantified as described in Kamimoto et al. (2012). Both ionophores abolish EDS5-GFP and VC-mediated SA export (negative retention) demonstrating that both EDS5 and vector control (background) SA transport is dependent on a electrochemical proton gradient. Significant differences (student’s t-test; p<0.05) of means ± SE (n = 4) to vector (Control) or solvent controls (Solvent) are indicated by one or two asterisks, respectively.

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Supplementary Fig. 2. Complementation analysis of transgenic lines. Complementation of

the EDS5 function was tested by the competence to induce SA after UV exposure of the

transformed plants

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. Total SA accumulation is shown. Significant differences (student’s t-test;

p<0.05) of means ± SD (n = 4) to non UV-induced plants are indicated by asterisks.

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