HAL Id: hal-02833559
https://hal.inrae.fr/hal-02833559
Submitted on 7 Jun 2020
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Human epididymal secretome and proteome:
comparative analysis with other mammals
Jean-Louis Dacheux, Maya Belghazi, Y. Lanson, Françoise Dacheux
To cite this version:
Jean-Louis Dacheux, Maya Belghazi, Y. Lanson, Françoise Dacheux. Human epididymal secretome
and proteome: comparative analysis with other mammals. Symposium on ”Post-Meiotic Approaches
to Male Contraception”, Jun 2005, Hong Kong, China. n.p. �hal-02833559�
HUMAN EPIDIDYMAL SECRETOME AND PROTEOME: COMPARATIVE ANALYSIS WITH OTHER MAMMALS.
Jean-Louis Dacheux
(1)Maya Belghazi
(2), Yves Lanson
(3), Françoise Dacheux
(1)
(1) UMR INRA-CNRS 6175, France-37380 Nouzilly (2) Service Urologie CHUR, 37000 Tours,
(3) Service de Spectrométrie de Masse pour la Protéomique, INRA, F-37380 Nouzilly.
In all mammals studied, testicular spermatozoa do not develop the ability to fertilize an ovum until they transit the epididymis. In this organ, several successive differentiations occur to the gametes, particularly in the lipid and protein composition of their plasma membrane inducing important changes in the characteristics of the sperm surface. These sperm modifications occur always in the epididymal environment which regulated continuously, particularly in protein composition.
Synchrony between changes in sperm and the epididymal milieu indicate that they are associated.
We present a comparative analysis, of human and several other mammals, in the sequential changes in the protein composition of the epididymal luminal fluid and in the synthetic activity of the epididymal epithelium.
The secreted proteins were determined by incubating tubules in vitro and the luminal proteins were determined using luminal fluid collected by flushing lengths of tubules, and the preparations were analysed by two dimensional electrophoresis and nano LC MS/MS.
In most species, such as in porcine, bovine, equine and rodent, epididymal activity is highly regionalized both in the secretory activity of the epithelium and the protein composition of the luminal fluid. However, in humans, this regionalization is present, but the number of sequential changes along the epididymis is reduced. Several proteins are found in all the species studied but their concentrations along the tubule are species specific.
In conclusion, in all the species studied, the protein composition of the luminal fluid
changed significantly according to the region of the epididymis. However in humans,
there were only minor changes in the major proteins secreted. It is suggested that
specificity of protein secretion could be related to the difference between species in
the location in the epididymis where the sperm become fertile.
