• Aucun résultat trouvé

Olfactory receptors in pulmonary arterial hypertension: A novel pathway of vascular remodeling?

N/A
N/A
Protected

Academic year: 2021

Partager "Olfactory receptors in pulmonary arterial hypertension: A novel pathway of vascular remodeling?"

Copied!
2
0
0

Texte intégral

(1)

HAL Id: hal-01343640

https://hal.archives-ouvertes.fr/hal-01343640

Submitted on 3 Jun 2020

HAL is a multi-disciplinary open access

archive for the deposit and dissemination of sci-entific research documents, whether they are pub-lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

Olfactory receptors in pulmonary arterial hypertension:

A novel pathway of vascular remodeling?

Audrey Courboulin, Guenhaël Sanz, Sandra Breuils-Bonnet, Marceau

Quatredeniers, Matthieu Vocelle, Mathilde Lafond, Frédéric Perros, Sebastien

Bonnet, Edith Pajot, Marc Humbert, et al.

To cite this version:

Audrey Courboulin, Guenhaël Sanz, Sandra Breuils-Bonnet, Marceau Quatredeniers, Matthieu Vo-celle, et al.. Olfactory receptors in pulmonary arterial hypertension: A novel pathway of vascular remodeling?. International Congress of the European Respiratory Society, Sep 2015, Amsterdam, Netherlands. European Respiratory Journal, 46 (suppl 59), 2015, European Respiratory Journal. �10.1183/13993003.congress-2015.PA2431�. �hal-01343640�

(2)

Olfactory receptors in pulmonary arterial

hypertension: A novel pathway of vascular

remodeling?

Audrey Courboulin, Guenhaël Sanz, Sandra Breuils-Bonnet, Marceau Quatredeniers, Matthieu Vocelle, Mathilde Lafond, Frédéric Perros, Sébastien Bonnet, Edith Pajot-Augy, Marc Humbert, Sylvia Cohen-Kaminsky

DOI: 10.1183/13993003.congress-2015.PA2431 Published 1 September 2015  

Abstract

 

Pulmonary arterial hypertension (PAH) is due to progressive obstruction of pulmonary arteries, thus leading to right heart failure and death. Breath volatile organic compounds (VOCs) can discriminate PAH and controls. Thus, a unique breath-print of PAH is detected using an artificial nose. VOCs target olfactory receptors (ORs) in olfaction. Interestingly, ORs are detected in peripheral tissues not related to olfaction and their deregulation is associated to cancer development. PSGR, encoded by the OR51E2 gene, is one of the ORs. Because

vascular cells in PAH exhibit properties of cancer cells, we propose the ground-breaking hypothesis that ORs participate to vascular remodeling leading to PAH.

Thus we aim to determine whether a deregulated expression and function of the pulmonary vascular PSGR could participate to the pathological phenotype of vascular cells, and its potential use as a novel therapeutic target in PAH.

PSGR gene and protein expression were assessed in total lung, distal pulmonary arteries and PASMCs from PAH patients compared to controls using qRT-PCR and western blot. We evaluated proliferation (Ki67) and apoptosis (TMRM) after siRNA-directed silencing of PSGR expression in PASMCs.

We demonstrate that PSGR expression is significantly increased (50%) in PASMC, in total lung and isolated pulmonary arteries from PAH patients compared to controls. PSGR silencing in PAH-PASMCs decreased both cell proliferation (20%) and resistance to apoptosis (25%).

This deregulated OR expression in PAH PASMCs opens a new avenue in PAH

pathophysiology. The whole spectrum of ORs is currently investigated using microarrays and deregulated ORs will be evaluated both in vitro and in vivo.

Références

Documents relatifs

PAH diagnosis is based on right heart catheterisation (RHC) [1], showing a mean pulmonary arterial pressure (mPAP) above 25 mmHg, associated with pulmonary

This retrospective analysis of the French medical information PMSI-MSO database aimed to describe incident cases of patients with pulmonary arterial hypertension hospital- ised

Attenuated hepatocyte dedifferentiation was demonstrated by reduced expression of N-Cadherin and Collagen 1α1 mRNA, as well as sustained E-Cadherin ex- pression when cells were

However reversed projection angles, a global constant shift of the projection angles or a global sinuso¨ıdal shift of the line index at each projection angle lead to

L’accès à ce site Web et l’utilisation de son contenu sont assujettis aux conditions présentées dans le site LISEZ CES CONDITIONS ATTENTIVEMENT AVANT D’UTILISER CE SITE WEB.

En allant à la rencontre de ces sujets empêtrés dans leur corps, aux prises avec les vestiges encombrants de leur sexualité, tentant d’accéder à une parentalité, nous nous

Thus, acquisition and communication data policies of a WSN node reflect both the current node state (for example, the node energy level) and the phenomenon state.. All of these