Supplementary Data
Modelling Nanoparticle-Alveolar Epithelial Cell Interactions Under Breathing Conditions Using Captive Bubble
Surfactometry
David Schürch1,2, Dimitri Vanhecke1, Martin J. D. Clift1, David Raemy1,2, Dorleta Jimenez de Aberasturi3, Wolfgang
Parak3, Peter Gehr2, Alke Petri-Fink1, and Barbara Rothen-Rutishauser1,4 *
1
Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
2
Institute of Anatomy, University of Bern, Bern, Switzerland
3
Philipps Universität Marburg, Fachbereich Physik, Marburg, Germany
4
Respiratory Medicine, Bern University Hospital, Bern, Switzerland
*Corresponding Author:
Prof. Barbara Rothen-Ruthishauser
Adolphe Merkle Institute
Université de Fribourg Route de l'ancienne Papeterie CP 209
CH-1723 Marly 1
Phone +41 26 300 95 02
Email: [email protected]
1
Published in /DQJPXLU ± which should be cited to refer to this work.
Adsorption data for CBS-cell experiments
Ji (mN/m)
J5s (mN/m) Jpost5s (mN/m)48.1r 3.0 27.4 r 2.1 27.8 r 1.7
Table S1: Adsorption data for CBS-cell experiments.Ji: initial surface tension,J5s: surface tension 5 seconds after adding surfactant + Au-NPs, Jpost5s: surface tension 5 seconds after rapid expansion of interface. Values are means r SD for 6 independent experiments.
Dynamic cycling data for CBS-cell experiments under dynamic conditions
Jmin(mN/m) 'Area (% max)
cycle1 14.4 r 7.6 54 r 12 cycle2 6.2 r 2.6 42 r 5 cycle5 1.7 r 0.8 22 r 4 cycle20 1.5 r 0.3 15 r 2 cycle80 1.4r 0.2 14 r 2 cycle200 1.4r 0.3 16 r 3
Table S2: Dynamic cycling data for CBS-cell experiments.Jmin: minimum surface tension during compression/expansion cycle.'Area (% max): Area change to minimum surface tension during compression/expansion cycle, expressed as % of maximum area during cycle. Values are means r SD for 5 independent experiments.
2
Supplementary Figure S1. Visualization of Au-NPs in epithelial cell layer with LSM. Fluorescence due Au-NPs appears as red points. Images are shown from 5 separate experiments with particle events indicated by arrow a) b) Cell layer after dynamic CBS-cell protocol, applied Au-NP conc. of 28 nM. c) d) Cell layer after dynamic CBS-cell protocol, applied NP conc. of 40 nM). e) Negative control: Cell layer after dynamic CBS-cell protocol with no Au-NPs applied
Video (compexpans.avi): Compression/expansion of air-liquid-cellular interface (dynamic cycling)
a)
b)
c)
e)
d)
3
