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In vitro anticoccidial activity of olive pulp (Olea europaea L. var. chemlal) extract against Eimeria oocysts in broiler chickens

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In vitro anticoccidial activity of olive pulp (Olea europaea L. var. chemlal) extract against Eimeria oocysts in broiler chickens

Authors

Nedjima Debbou-Iouknane, Cristina Nerín, Meriem Amrane, Menana Ghemghar, Khodir Madani, Abdelhanine Ayad

Publication date 2019/12 Journal Acta parasitologica Volume 64 Issue 4 Pages 887-897 Publisher

Springer International Publishing Description

Aim

The objective of the present study was to investigate in vitro anticoccidial effect of olive pulp (Olea europaea L var. Chemlal) extract on the destruction of Eimeria spp. oocysts isolated from infected chickens naturally.

Materials and methods

The olive pulp (OP) powder was stirred manually in aqueous ethanol in preparation for extraction using the microwave-assisted extraction system. The identification of the phenolic compounds was obtained by ultra-high-performance liquid

chromatography–mass spectrometry with electrospray ionisation (HPLC–ESI–MS). The treatment of Eimeria oocyst with OP extract and standard compounds (quercetin and oleuropein) leads to their lysis as shown by the release of substances absorbing at 273 nm.

Results

Our results showed that the maximum number of reduced oocysts was recorded after …

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