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RANKL directly induces bone morphogenetic protein-2 expression in RANK-expressing POS-1 osteosarcoma cells.

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Figure 1. Micro-CT scans of mouse femora 4 weeks after implantation with POS-1 osteosarcoma as compared to controls
Figure 3. In vitro characterization of osteogenic properties of POS-1 cells.
Figure 5. RANKL induces BMP-2 expression in POS-1 cells. A, relative BMP-2 mRNA levels were measured by semi-quantitative RT-PCR in POS-1 cells treated for 6 h in the presence of increasing concentrations of human RANKL
Figure 7. RANKL-induced BMP-2 expression in POS-1 cells is inhibited by soluble RANK. At 70-80% confluence, pOS-1 cells were treated for 6 h with 100 ng/ml hRANKL that was first pre-incubated for 1 h with 400 ng/ml human soluble RANK (2-fold molar excess)

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