• Aucun résultat trouvé

DIAGNOSTIC POTENTIAL OF MIRNAS IN PARATHYROID CARCINOMAS

N/A
N/A
Protected

Academic year: 2021

Partager "DIAGNOSTIC POTENTIAL OF MIRNAS IN PARATHYROID CARCINOMAS"

Copied!
1
0
0

Texte intégral

(1)

DIAGNOSTIC POTENTIAL OF MIRNAS IN PARATHYROID CARCINOMAS

DIAGNOSTIC POTENTIAL OF MIRNAS IN PARATHYROID CARCINOMAS

DIAGNOSTIC POTENTIAL OF MIRNAS IN PARATHYROID CARCINOMAS

DIAGNOSTIC POTENTIAL OF MIRNAS IN PARATHYROID CARCINOMAS

A. Ladang, Y. Seynaeve, E Cavalier

Department of Clinical Chemistry, ULiège and CHU of Liège, Belgium

Parathyroid carcinoma is a rare endocrine cancer with a poor prognosis. Additionally, diagnostic tools that discriminate parathyroid carcinomas from adenomas are insufficient although essential in the

therapeutic scheme. Previous reports have identified, in parathyroid biopsies, several potential biomarkers that are differentially expressed in parathyroid carcinomas compared to adenomas. In this study,

we investigate the expression profile of those previously reported miRNAs in sera of patients suffering from parathyroid carcinoma.

Our cohort is composed of sera from 10 parathyroid carcinomas, 12 parathyroid adenomas and 11 healthy subjects. 9 miRNAs were selected based on literature namely: 222-3p, 30b-5p,

miR-139-5p, miR-517c-3p, miR-126-5p, miR-26b-5p, miR296-5p, miR-503-5p and miR-30e-5p. miRNA were extracted using miRNeasy Serum/Plasma Advanced Kit and cDNA synthetized with miRCURY

LNA RT Kit. miRNA expression profile was quantified by real-time qPCR assays Lightcycler (Roche°) using miRCURY LNA SYBR Green PCR Kit.

Introduction

Introduction

Introduction

Introduction::::

Material

Material

Material

Material and Methods

and Methods

and Methods::::

and Methods

Results

Results

Results

Results::::

Conclusions:

Conclusions:

Conclusions:

Conclusions:

This study identifies miR-30e-5p as potential serum biomarker for parathyroid carcinomas. It also shows that previously reported miRNA identified in parathyroid carcinoma

biopsies are not inapplicable in serum matrix. Given the frequency of the disease, we believe that those results are interesting but should be confirmed by other studies.

@

@

Fig 2. miRNA expression profile: miRNA expression profile: miRNA expression profile: miRNA expression profile: No significant differences were found for miR-222-3p, miR-30b-5p, miR-139-5p, miR-126-5p and miR-26b-5p. miR-517c-3p, miR296-5p, miR-503-5p were not expressed in all samples. Relative expression of miRNA are expressed in Ct values normalized to miR-484 CT values (PCa: Parathyroid carcinomas; PAd: Parathyroid adenomas; VS:

healthy subjects).

Fig 3. miRmiRmiR----30emiR30e30e30e----5p expression: 5p expression: 5p expression: (Upper graph): miR-30e-5p was significantly differentially 5p expression: expressed in parathyroid carcinomas compared to the other subpopulations. (Lower graph): A significant logarithmic correlation was observed between miR-30e-5p and PTH values. Relative expression of miRNA are expressed in Ct values normalized to miR-484 CT values (PCa:

Parathyroid carcinomas; PAd: Parathyroid adenomas; VS: healthy subjects).

@

Parathyroid carcinomas Parathyroid adenomas Healthy subjects

n 3♀ - 7♂ 5♀ - 7♂ 5♀ - 6♂ Age (years) 49,3 ± 13,5 61,4 ± 11,3 58 ± 19,7 Calcium (mmol/L) 3,58 ± 0,5 2,91 ± 0,4 2,4 ± 0,1 Phosphates (mmol/L) 0,654 ± 0,2 0,76 ± 0,2 0,99 ± 0,2 PTH (ng/L) 1211 ± 1563,9 86,7 ± 75,6 17,8 ± 5,7 25-OH-VTD (ng/mL) 12,2 ± 6,7 32,6 ± 7,3 29,2 ± 7,2 Creatinine (mg/mL) 14,6 ± 3,7 10,96 ± 3,7 10,8 ± 3,1 GFR 44,6 ± 15,6 56,7 ± 6,9 54,4 ± 7,2

Fig 1. Demographic Demographic Demographic Demographic characteristics of the characteristics of the characteristics of the characteristics of the studied population. studied population. studied population. studied population. -1,5 -1,0 -0,5 0,0 0,5 1,0 1,5 2,0 10 100 1000 10000 miR-30e-5p (∆∆CT) P T H ( n g /L ) PAd VS PCa

Figure

Fig 2. miRNA expression profile:  miRNA expression profile:  miRNA expression profile:  miRNA expression profile: No significant differences were found for miR-222-3p, miR-30b-5p, miR-139-5p, miR-126-5p  and miR-26b-5p

Références

Documents relatifs

Training exercises are now frequently used in health disaster and emergency medicine to train.. first responders, 1 including the use of

[r]

However, the main noticeable differences were the increased miR-155-5p values for the loaded NP condition in respect to cells cultured with miRNA, suggesting ability

Des immunomarquages CD31 (cellules endothéliales) / SMA (cellules musculaires lisses) et CD31 (cellules endothéliales) / NG2 (péricytes) dans nos conditions

Stable inhibition of miR-145-5p in immortalized prostate PNT1A epithelial cells and in PC3 cancer cells led to increased TWIST1 expression and consequently to stemness

case in which ab is a closest pair of points in V (the base case for our induction)... Assume now that the inductive hypothesis holds for all pairs of points closer than

7-Relationship between applied vertical stress and change in void ratio for incremental and constant rate of loading tests... loading together with the significant

Formation of rosettes in as solidified aluminium alloys is related to liquid entrapment within the primary (Al) phase, which then undergoes large undercoolings for second