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Small-Angle x-ray scattering and crystallographic studies of arcelin-1: An insecticidal lectin-like glycoprotein fromPhaseolus vulgaris L

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Fig. 1. Sequence alignement of Lathyrus ochrus isolectin I (LoLI), erythroagglutinating (PHA-E), and leucoagglutinating (PHA-L) subunits of phytohemagglutinin, a -amylase inhibitor 1 ( a -AI1), and arcelin-1 (ARCE-1)
TABLE I. Conditions Used for Arcelin-1 SAXS Experiments* Buffer Added salt pH I(M) R g(Å 6 0.2 Å)TypeConc.(M) MES — 4.9 0.10 — MES NaSCN 0.3 4.9 0.40 — MES NaCl 0.3 4.9 0.40 28.6 MES Na citrate 0.3 4.9 0.52 28.8 MES NaSCN 1.0 4.9 1.10 28.7 MES (NH4) 2 SO 4 0.3 4.9 1.00 29.3 Na citrate — 5.0 0.15 28.8 Na citrate NaSCN 0.3 5.0 0.45 29.0 Na citrate (NH4) 2 SO 4 0.3 5.0 1.05 29.4 *All protein and buffers concentrations were 5 mg/ml and 0.1 M, respectively.
Fig. 2. Guinier plots of 5 mg/ml arcelin-1 solutions in (A) 0.1 M MES pH 4.9 (I 5 0.1 M), and in the same buffer supplemented with (B) 0.3 M sodium thiocyanate (I 5 0.4 M)
Fig. 3. Guinier plots of 5 mg/ml arcelin-1 solutions in (A) 0.1 M MES pH 5 4.9 and in the same buffer supplemented with (B) 1.0 M  so-dium thiocyanate (I 5 1.1 M)
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