Molecular studies on the densovirus of Mytbimna loreyi, MIDNV a candidate for biological control

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CHAPTER

IV :

RESULTS

ARTICLE

I

EXPRESSION STRATEGY OF DENSONUCLEOSIS

VIRUS FROMMYTHIMNA LOREY/

Cet article a dû être retiré en raison de restrictions liées au droit d’auteur.

Virology. 2004 Mar 1;320(1):181-9.

Fédière G, El-Far M, Li Y, Bergoin M, Tijssen P. Expression strategy of densonucleosis virus from Mythimna loreyi. Virology. 2004 Mar

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86 PREFACE

The densovirus MlDNV has been isolated and characterized with respect to host range in the mid 1990s by Dr. Gilles Fédière, who was then leading a research group at the "Institut de la recherche pour le développement (formerly ORSTOM, France)" at the Faculty of Agriculture ofCairo University. He obtained severa!partial and complete clones of the viral genome during his sahhatical year in our lahoratory ofiNRS - Institut Armand-Frappier (1997-1998) and sequenced up to 90o/o of the genome. I reconfrrmed the earlier sequence and obtained the remaining hairpin sequences that were stilllacking (Fig. 2A). 1 also carried out ali the bioassay tests and the transcription mapping (Fig 1. and Fig. 2B and 3, respectively). Dr. Yi Li contributed by helping in the construction and testing the baculovirus expression system to study the mechanism of viral protein production (Fig. 4).

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CHAPTER

VII :

RESULTS

ARTICLE

III

LACK OF INFECTIVITY OF VERTEBRATE CELLS BY

DENSOVIRUS FROM THE MAIZE WüRM

MYTHIMNA LOREY!

(MLDNV)

Cet article a dû être retiré en raison de restrictions liées au droit d’auteur.

El-Far M, Li Y, Fédière G, Abol-Ela S, Tijssen P. Lack of infection of vertebrate cells by the densovirus from the maize worm Mythimna loreyi (MlDNV).

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148

PREFACE

The innocuity of the densovirus has been debated for a long rime since earlier

reports claimed that these viroses could infect and transform mammalian cells in culture. A major part of my study was to verify the validity of these reports, we choose the

MlDNV as a relatively polyspecific virus but we have also used the GmDNV. The

availability of the infectious clones of both viroses provided us with more powerful

molecular tools to study both mammalian as well as insect cell systems. The experiments

were carried out by myself under the direction of Dr.Tijssen and the help of Dr. Li. Dr.

Gilles Fédière and Dr. Said Aboi-Ela from the Faculty of Agriculture, Cairo University,

have provided us with the original virus MlDNV preparations, as well by sorne other

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