Aggregation in Ladybeetles: From chemistry to behaviour

(1)

AGGREGATION IN

LADYBEETLES

From chemistry to behaviour

(2)

Delphine Durieux PhD Thesis

(2008-2012)

« Identifying the chemical mecanisms

gouverning aggregation behaviour in

(3)

Reduction of

Biodiversity

New fruit pest

Aggregation inside

human constructions

(4)

FIVE STEPS TO SELECT AN AGGREGATION SITE

(5)

FIVE STEPS TO SELECT AN AGGREGATION SITE

5

?

2) Landing on a macrosite

4) Searching for a microsite

3) Decision making

_{5) Settlement in a microsite}

(6)

DOES A CHEMICAL MARKING OCCUR DURING

THE AGGREGATION PROCESS ?

(7)

DOES A CHEMICAL MARKING OCCUR DURING

THE AGGREGATION PROCESS ?

1.  Do they mark the aggregation site ?

2.  Do they mark the way to the aggregation

site ?

3.  What is the chemical composition of the

marking ?

4.  Is the marking used from one year to the

other ?

5.  Does the composition of the marking

changes over time ?

6.  Is the aggregative behaviour specific to

winter conditions ?

(8)

(9)

1. DO THEY MARK THE AGGREGATION SITE ?

1

6

2 ₃

4

5 Multiple choice arena

Durieux D, Fischer C, Brostaux Y, Sloggett JJ, Deneubourg JL., Vandereycken A, Joie E, Wathelet JP, Lognay G, Haubruge E, Verheggen FJ(2012).

(10)

1. DO THEY MARK THE AGGREGATION SITE ?

1

6

2 ₃

4

5 8 hours later :

χ² : P < 0,001

Multiple choice arena

M ea n % a gr eg ated la dy beetl es Zones

(11)

1. DO THEY MARK THE AGGREGATION SITE ?

1

6

2 ₃

4

5 solvent

Multiple choice arena

(12)

1. DO THEY MARK THE AGGREGATION SITE ?

1

6

2 ₃

4

5 8 hours later :

χ² : P < 0,001

Multiple choice arena

(13)

2. DO THEY MARK THE WAY TO THE AGGREGATION SITE ?

Role of long-chain hydrocarbons in the aggregation behaviour of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae). Journal of Insect Physiology. 58, p. 801-807.

76%

66%

24%

34%

χ² : P < 0.001 (males) and P = 0.024 (females)

♂

♀

N umber of beetl es in ea ch bra nche

(14)

2. DO THEY MARK THE WAY TO THE AGGREGATION SITE ?

75%

77%

25%

_22%

χ² : P = 0.002 (males) and P = 0.001 (females)

♂

♀

N umber of beetl es in ea ch bra nche

(15)

Saturated hydrocarbons

Mono-unsaturated hydrocarbons

Di-unsaturated hydrocarbons

R

1

R

₂

R

₁

R

₂

R

1

R

₂

C

₂₃

C

₂₄

C

₂₅

:1

C

₂₇

:2

C

₂₇

C

₂₇

:1 C

29

:2

C

₂₉

C

₂₉

:1

Me-C

₂₉

C

₃₁

:1

C

₃₁

:2

C

₂₅

(16)

3. WHAT IS THE CHEMICAL COMPOSITION OF THE MARKING ?

(17)

1.  Do they mark the aggregation site ?

2.  Do they mark the way to the aggregation site ?

3.  What is the chemical composition of the marking ?

(18)

4. IS THE MARKING USED FROM ONE YEAR TO THE OTHER ?

(19)

4. IS THE MARKING USED FROM ONE YEAR TO THE OTHER ?

Durieux D., Fassotte B., Vanderplanck M., Brostaux Y., Fischer C., Lognay G., Haubruge E., Verheggen F.J.(2014). Is conspecific area marking a long-term external memory of previously colonized overwintering sites in Harmonia axyridis? Journal of Applied Entomology, 138:338-345

Beside a window

1st flour

Orientation South

solvent

1

6

2 ₃

4

5

(20)

4. IS THE MARKING USED FROM ONE YEAR TO THE OTHER ?

8 hours later :

1

6

2 ₃

4

5 Multiple choice arena

(21)

4. IS THE MARKING USED FROM ONE YEAR TO THE OTHER ?

Durieux D., Fassotte B., Vanderplanck M., Brostaux Y., Fischer C., Lognay G., Haubruge E., Verheggen F.J.(2014). Is conspecific area marking a long-term external memory of previously colonized overwintering sites in Harmonia axyridis? Journal of Applied Entomology, 138:338-345

C₂₃ C₂₄

C₂₅

C₂₇ C₂₉ Me-C29

Fresh marking

_{One year old marking}

(22)

(23)

5. DOES THE COMPOSITION OF THE MARKING CHANGES OVER TIME ?

(24)

5. DOES THE COMPOSITION OF THE MARKING CHANGES OVER TIME ?

(perMANOVA, P < 0.001)

From April to September

(outside)

From October to February

(aggregates)

March

(25)

5. DOES THE COMPOSITION OF THE MARKING CHANGES OVER TIME ?

Durieux D., Fassotte B., Vanderplanck M., Deneubourg JL, Fischer C, Lognay G., Haubruge E., Verheggen F.J.(2013). Substrate marking by an invasive ladybeetle: Seasonal changes in hydrocarbon composition and behaviora responses PLOSOne

(26)

6. IS THE AGGREGATIVE BEHAVIOUR SPECIFIC TO WINTER CONDITIONS ?

(27)

6. IS THE AGGREGATIVE BEHAVIOUR SPECIFIC TO WINTER CONDITIONS ?

Durieux D., Deneubourg J.L., Brostaux Y., Vandereycken A., Joie E., Haubruge E., Verheggen F.J. (2014). Aggregation behavior of Harmonia axyridis in non-wintering conditions. Insect Science

(28)

6. IS THE AGGREGATIVE BEHAVIOUR SPECIFIC TO WINTER CONDITIONS ?

M ea n pr opo rti on of shel ter ed la dy beetl es Time (min)

(29)

6. IS THE AGGREGATIVE BEHAVIOUR SPECIFIC TO WINTER CONDITIONS ?

Durieux D., Deneubourg J.L., Brostaux Y., Vandereycken A., Joie E., Haubruge E., Verheggen F.J. (2014). Aggregation behavior of Harmonia axyridis in non-wintering conditions. Insect Science

Aggregation in Ladybeetles: From chemistry to behaviour

AGGREGATION IN

LADYBEETLES

From chemistry to behaviour

Delphine Durieux PhD Thesis

(2008-2012)

« Identifying the chemical mecanisms

gouverning aggregation behaviour in

Reduction of

Biodiversity

New fruit pest

Aggregation inside

human constructions

FIVE STEPS TO SELECT AN AGGREGATION SITE

FIVE STEPS TO SELECT AN AGGREGATION SITE

?

2) Landing on a macrosite

4) Searching for a microsite

3) Decision making

5) Settlement in a microsite

DOES A CHEMICAL MARKING OCCUR DURING

THE AGGREGATION PROCESS ?

DOES A CHEMICAL MARKING OCCUR DURING

THE AGGREGATION PROCESS ?

1. Do they mark the aggregation site ?

2. Do they mark the way to the aggregation

site ?

3. What is the chemical composition of the

marking ?

4. Is the marking used from one year to the

other ?

5. Does the composition of the marking

changes over time ?

6. Is the aggregative behaviour specific to

winter conditions ?

1. DO THEY MARK THE AGGREGATION SITE ?

1

6

2

3

4

5

Multiple choice arena

1. DO THEY MARK THE AGGREGATION SITE ?

1

6

2

3

4

5

8 hours later :

χ² : P < 0,001

Multiple choice arena

1. DO THEY MARK THE AGGREGATION SITE ?

1

6

2

3

4

5

solvent

Multiple choice arena

1. DO THEY MARK THE AGGREGATION SITE ?

1

6

2

3

4

5

8 hours later :

χ² : P < 0,001

Multiple choice arena

2. DO THEY MARK THE WAY TO THE AGGREGATION SITE ?

76%

66%

24%

34%

χ² : P < 0.001 (males) and P = 0.024 (females)

♂

♀

_{5) Settlement in a microsite}

1.  Do they mark the aggregation site ?

2.  Do they mark the way to the aggregation

3.  What is the chemical composition of the

4.  Is the marking used from one year to the

5.  Does the composition of the marking

6.  Is the aggregative behaviour specific to

₃

₃

₃

₃

_22%

1.  Do they mark the aggregation site ?

2.  Do they mark the way to the aggregation site ?

3.  What is the chemical composition of the marking ?

₃

₃

_{One year old marking}