Prebiotics are used to modulate the intestinal microbiota with the possible effect of reducing acute gastroenteritis induced by Salmonella infection.
Objective: Prebiotic effects of nondigestible carbohydrates (NDC) on the microbiota in presence of S. Typhimurium using an in vitro gas fermentation model.
Introduction
Results
Materials & Methods
Salmonella in fermentation medium
Log cfu/ml 2,5 3,5 4,5 5,5 12h 24h
No effects of the NDC on Salmonella populations
5,9 6,4
Log cfu/ml
6,3
Log cfu/ml
Prebiotic effects of novel nondigestible carbohydrates on bacterial
community in presence of
S.
Typhimurium in an
in vitro
system
Tran T.H.T.1,3, Bindelle J.1, Portetelle D.2, Thewis A.1, Boudry C.1
1 Animal Science Unit and 2 Microbial Biology Unit, Gembloux Agro-Bio Tech, University of Liege, Belgium 3 Wallonie-Bruxelles International, Brussels, Belgium
Even if no effects of the NDC were observed on Salmonella populations, IMO showed the highest prebiotic potential.
Conclusion
in fermentation medium 4,4 4,9 5,4 5,9 6h 12h 24h 72h IMO inuline XOS POS cellobio gluconat blanc inoculum es e Lactobacillus 3,3 4,3 5,3 6h 12h 24h 72h BifidobacteriumIMO: high and stable Lactobacillus and Bifidobacterium concentration & Fermentation broth sampling after 6, 12, 24 and 72h Microbiota quantification by qPCR Fresh faeces Nutritive buffer NDC: Inulin Isomaltooligosaccharides (IMO) Xylooligosaccharides (XOS) Pecticoligosaccharides (POS) Cellobiose Gluconate Mucin-covered microcosm (MCM) S. Typhimurium 1,2 x 109CFU/ml Salmonella Lactobacillus Bifidobacterium Clostridium Cluster I Bacteroides