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Tfam Knockdown Results in Reduction of mtDNA Copy Number, OXPHOS Deficiency and Abnormalities in Zebrafish Embryos

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Academic year: 2021

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FIGURE 1 | Knockdown of TFAM in zebrafish embryos. Zebrafish embryos injected with indicated amount of either Control-morpholino (Ctrl-MO) or tfam splice-morpholino tfam splice-MO at 1 hpf and analyzed at 4 dpf
FIGURE 2 | Microscopy of zebrafish embryos following TFAM knockdown. (A–H) Microscopic analysis following serial HE-staining of zebrafish embryos at 4 dpf, which were injected at 1 hpf with either 2 ng tfam splice-MO (B–E) or 2 ng Ctrl-MO, (A,C,E,G) n = 13
TABLE 2 | Mitochondria-related GO biological processes.
FIGURE 3 | Schematic overview of alterations in tfam splice-MO zebrafish at 4dpf. The arrow indicates if a process is upregulated (green) or down-regulated (red).

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