• Aucun résultat trouvé

Quantitative Analysis of the Relative Abundance Changes Observed in Type I, III and V Collagen after Suspensory Ligament Injuries in Horses

N/A
N/A
Protected

Academic year: 2021

Partager "Quantitative Analysis of the Relative Abundance Changes Observed in Type I, III and V Collagen after Suspensory Ligament Injuries in Horses"

Copied!
1
0
0

Texte intégral

(1)

Topic: Musculo-skeletal system

ORAL PRESENTATION

Quantitative Analysis of the Relative Abundance Changes Observed in Type I, III and V Collagen after Suspensory Ligament Injuries in Horses

Mohamad Khir Shikh Alsook1 , Etienne Baise2, Joelle Piret3, Nassim Moula4, Moustafa Salouci1, Nadine Antoine3, Jean-Marie Denoix5 and Annick Gabriel1.

1

Anatomy Unit, FARAH Research Center & Faculty of Veterinary Medicine, University of

Liège, Liège, Belgium

2

FARAH Research Center & Faculty of Veterinary Medicine, University of Liège, Liège,

Belgium.

3

Histology Unit, FARAH Research Center & Faculty of Veterinary Medicine, University of

Liège, Liège, Belgium.

4

Sustainable Animal Production Unit,

FARAH Research Center & Faculty of Veterinary

Medicine, University of Liège, Liège, Belgium

5

CIRALE -14430 Goustranville, Université Paris-Est, Ecole Nationale Vétérinaire d’Alfort,

Paris, France

Introduction: The equine suspensory ligament (SL) injuries are an important cause of lameness in horses. Quantification of the relative abundance of the constitutive collagen types is essential to

investigate the differences between normal and injured tissues. The aim of this original study was to precisely determine the different collagen ratios both in injured and normal SL. This approach is a rational step towards understanding the intimate mechanisms of SL repair and regeneration at the molecular and histological levels.

Methods: Five Warmblood horses with visible signs of injury in only one forelimb SL were selected. Specimens were obtained from the central core of lesions in damaged SLs and from the corresponding regions in other healthy SLs. Collagen types I, III and V were purified by differential salt precipitation after collagen extraction with acetic acid containing pepsin and they were examined by transmission electron microscopy. The bands corresponding to types I, III, and V collagen were assessed by densitometry after SDS-PAGE. SAS software (SAS Institute 2001) was used for all statistical analyses.

Results: Based on ultrastructural observations, purified fibers of types I, III, and V collagens have been identified. The relative proportions of type III and type V collagen were significantly higher in the specimens from damaged tissue compared with specimens from normal tissue (P < 0.001). These changes were concomitant with significant decrease in type I collagen in the injured tissue (P < 0.001). Conclusions: Our study showed that after SL injury, the relative abundance of the different collagen types is modified. These changes are the molecular hallmark of a decrease in tissue quality and mechanical properties of the ligament. It lays down the bases of subsequent researches on the tissue regeneration that may lead to the development of new treatment strategies for damaged tissues, particularly in the equine SL injuries.

Références

Documents relatifs

The Moira Mounds, small cold-water coral banks in the Porcupine Seabight, NE Atlantic: Part A—an early stage growth phase for future coral carbonate mounds. Marine Geology,

En cas de réutilisation prévue dans un autre pays, il appartient à chaque utilisateur de vérifier la conformité de son projet avec le droit de ce pays.. 6/ L'utilisateur s'engage

2014 The role of the bound hole-phonon interaction in the phonon conductivity of lightly doped p-type III-V semiconductors such as p-GaSb, p-InSb, p-AlSb, p-GaP as well

In France, a game called carom is played, where only 3 balls are used on a pocket-less table, and the player must attempt to contact both object balls with the cue ball.. In the

Ce partenariat belgo-ivoirien a été le catalyseur d’un projet de thèse de doctorat original visant à évaluer l’impact de différentes stratégies (non basées sur

The aim of the experiment is to use the model presented in section II to learn arm contexts, according to the different inputs (grip, localisation, arm position,...) so that the

Juanes (2014), Coupled multiphase flow and poromechanics: A computational model of pore pressure effects on fault slip and earthquake triggering, Water Resour... disposal of

Nous avons volontairement limit´e notre ´etude applica- de cette th`ese ´etait de proposer un ensemble d’outils pour la mod´elisation et la r´esolution tive aux probl`emes