0 2 4 6 2000 3000 time pred t Chemical DMSO POP 1 0.075 0.080 0.085 0.090 0.095 0.100 Concentration 1 2 3 4 2000 3000 time pred t Chemical DMSO POP 1 0.075 0.080 0.085 0.090 0.095 0.100 Concentration
METHODS
Realistic
Persistent Organic Pollutant mixture
has
Deleterious effects
in zebrafish larvae
Neuromast counting
GUERRERO-LIMON, Gustavo
1, CHRISTOU, M.
2, DOAN, T. Q.
3, MULLER, M
1.
Malformations and heartbeat rate
This project has received funding from the European Union’s Horizon 2020 research and
innovation programme under the Marie
Skłodowska-Curie grant agreement No. 722634.
Behavior
Laboratory for Organogenesis and Regeneration
BACKGROUND
The POP mix: health-relevant mixture of Persistent Organic Pollutant based on the highest levels of POPs occurring in Scandinavian blood and breast milk. We have studied its effects on zebrafish larvae development.
Alternative methods to assess the effects of a realistic Persistent Organic Pollutant
mixture (POP Mix) following a chronic exposure in zebrafish larvae (Danio rerio)
CONCLUSIONS
Significant acute responses observed:
Ø Underdeveloped (Non-inflated swim
bladder) even at 75-fold blood level.
Ø Edemas found in >90% of the
population in some cases.
Ø Deformed eyes in a remarkable
dose-dependent manner.
Ø Heartbeat rate was also disrupted.
Ø Larval behavior was affected: slightly
lower activity in dark phases, significantly increased activity in light phases.
Ø In contrast, no significant deleterious effects in the lateral line system (neuromast counts).
We observed several deleterious effects upon exposure of developing zebrafish larvae to the POP mix. The doses tested (75-250-fold mean blood levels) could be found in humans under specific circumstances and events. Since a cocktail of pollutants was used, further analyses are a most-do.
Control Group
POP mix (250x)
**** **** **** **** **** **** **** **** **** ** ** ** p value: **** = <0.0001; ** = <0.001 Sw im m in g spe ed Di st anc e
Dose-range finding
Affiliations1. Laboratory for organogenesis and regeneration, GIGA-I3, University of Liège. Belgium 2. Norwegian University of Life Sciences, Production Animal Clinical Sciences. Norway
3. Laboratory of food Analysis FARAH-Veterinary Public Health, University of Liège. Belgium Contact:
g.guerrero@uliege.be
RESULTS
Control (DMSO 0.1%)
POP 1 (75-fold blood level)
POP 2 (125-fold blood level)
POP 3 (250-fold blood level)
à
Dark phase
Light phase
Dark phase
Light phase
____
Control (DMSO 0.1%)
--- POP 1 (75-fold blood level)
0 200 400 600 800 1000 0.0 0.2 0.4 0.6 0.8 1.0 Treatment % Su rvi va l LC50 386x (fold blood level) 0 200 400 600 800 1000 Treatment Su rv iv al % 0 20 40 60 80 100
