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Sustained correction of B-cell development and function in a murine model of X-linked agammaglobulinemia (XLA) using retroviral-mediated gene transfer

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Academic year: 2021

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Figure 1. Transduction of 5FU-treated BM with the MBS vector. (A) Structure of the MSCV-huBtk-SAR (MBS) retroviral vector
Figure 3A illustrates the pre-B-developmental block in Btk-/
Figure 3. Reconstitution of B-lineage development in MBS-treated BtkTec ⴚ / ⴚ mice. BM, splenocytes, peripheral blood, and peritoneal cells were harvested from BtkTec ⫺/⫺ recipient mice 3 to 5 months after transplantation, evaluated by flow cytometry, and
Figure 4. Reconstitution of B-cell numbers in MBS-treated mice. Total numbers for B-cell subsets, based on the developmental schema of Li and colleagues, 48 were calculated using flow cytometry percentages and total cell counts
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